Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Is that bacteria dead yet?

01.07.2013
Nano and laser technology packed into small device tests antibiotic treatment in minutes

Researchers at EPFL have built a matchbox-sized device that can test for the presence of bacteria in a couple of minutes, instead of up to several weeks. A nano-lever vibrates in the presence of bacterial activity, while a laser reads the vibration and translates it into an electrical signal that can be easily read—the absence of a signal signifies the absence of bacteria.

Thanks to this method, it is quick and easy to determine if a bacteria has been effectively treated by an antibiotic, a crucial medical tool especially for resistant strains. Easily used in clinics, it could also prove useful for testing chemotherapy treatment. The research is published in the latest issue of Nature Nanotechnology.

"This method is fast and accurate. And it can be a precious tool for both doctors looking for the right dosage of antibiotics and for researchers to determine which treatments are the most effective," explains Giovanni Dietler.

Laser and nanotechnology read the bacteria's metabolic activity

It currently takes a long time to measure a bacterial infection's response to antibiotic treatment. Clinicians must culture the bacteria and then observe its growth, sometimes for almost a month, as is the case with tuberculosis, in order to determine if the treatment has been effective.

Thanks to advances in laser and optical technology, the EPFL team of physicists has reduced this time to a couple of minutes. To do so, Giovanni Dietler, Sandor Kasas and Giovanni Longo have exploited the microscopic movements of a bacterium's metabolism.

These vital signs are almost unperceivable. In order to test for them, the researchers place the bacteria on an extremely sensitive measuring device that vibrates a small lever—only slightly thicker than a strand of hair—in the presence of certain activity. The lever then vibrates under the metabolic activity of the germs. These infinitely small oscillations, on the order of one millionth of a millimeter, determine the presence or absence of the bacteria.

To measure these vibrations, the researchers project a laser onto the lever. The light is then reflected back and the signal is converted into an electrical current to be interpreted by the clinician or researcher. When the electrical current is a flat line, one knows that the bacteria are all dead; it is as easy to read as an electrocardiogram.

A promising method for cancer treatment

The researchers have miniaturized the tool—it is currently the size of a matchbox. "By joining our tool with a piezoelectric device instead of a laser, we could further reduce its size to the size of a microchip," says Giovanni Dietler. They could then be combined together to test a series of antibiotics on one strain in only a couple of minutes.

The researchers are currently evaluating the tool's potential in other fields, notably oncology. They are looking into measuring the metabolism of tumor cells that have been exposed to cancer treatment to evaluate the efficiency of the treatment. "If our method also works in this field, we really have a precious tool on our hands that can allow us to develop new treatments and also test both quickly and simply how the patient is reacting to the cancer treatment," says Sandor Kasas.

Lionel Pousaz | EurekAlert!
Further information:
http://www.epfl.ch

More articles from Life Sciences:

nachricht Water world
20.11.2017 | Washington University in St. Louis

nachricht Carefully crafted light pulses control neuron activity
20.11.2017 | University of Illinois at Urbana-Champaign

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: A “cosmic snake” reveals the structure of remote galaxies

The formation of stars in distant galaxies is still largely unexplored. For the first time, astron-omers at the University of Geneva have now been able to closely observe a star system six billion light-years away. In doing so, they are confirming earlier simulations made by the University of Zurich. One special effect is made possible by the multiple reflections of images that run through the cosmos like a snake.

Today, astronomers have a pretty accurate idea of how stars were formed in the recent cosmic past. But do these laws also apply to older galaxies? For around a...

Im Focus: Visual intelligence is not the same as IQ

Just because someone is smart and well-motivated doesn't mean he or she can learn the visual skills needed to excel at tasks like matching fingerprints, interpreting medical X-rays, keeping track of aircraft on radar displays or forensic face matching.

That is the implication of a new study which shows for the first time that there is a broad range of differences in people's visual ability and that these...

Im Focus: Novel Nano-CT device creates high-resolution 3D-X-rays of tiny velvet worm legs

Computer Tomography (CT) is a standard procedure in hospitals, but so far, the technology has not been suitable for imaging extremely small objects. In PNAS, a team from the Technical University of Munich (TUM) describes a Nano-CT device that creates three-dimensional x-ray images at resolutions up to 100 nanometers. The first test application: Together with colleagues from the University of Kassel and Helmholtz-Zentrum Geesthacht the researchers analyzed the locomotory system of a velvet worm.

During a CT analysis, the object under investigation is x-rayed and a detector measures the respective amount of radiation absorbed from various angles....

Im Focus: Researchers Develop Data Bus for Quantum Computer

The quantum world is fragile; error correction codes are needed to protect the information stored in a quantum object from the deteriorating effects of noise. Quantum physicists in Innsbruck have developed a protocol to pass quantum information between differently encoded building blocks of a future quantum computer, such as processors and memories. Scientists may use this protocol in the future to build a data bus for quantum computers. The researchers have published their work in the journal Nature Communications.

Future quantum computers will be able to solve problems where conventional computers fail today. We are still far away from any large-scale implementation,...

Im Focus: Wrinkles give heat a jolt in pillared graphene

Rice University researchers test 3-D carbon nanostructures' thermal transport abilities

Pillared graphene would transfer heat better if the theoretical material had a few asymmetric junctions that caused wrinkles, according to Rice University...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Ecology Across Borders: International conference brings together 1,500 ecologists

15.11.2017 | Event News

Road into laboratory: Users discuss biaxial fatigue-testing for car and truck wheel

15.11.2017 | Event News

#Berlin5GWeek: The right network for Industry 4.0

30.10.2017 | Event News

 
Latest News

Antarctic landscape insights keep ice loss forecasts on the radar

20.11.2017 | Earth Sciences

Filling the gap: High-latitude volcanic eruptions also have global impact

20.11.2017 | Earth Sciences

Water world

20.11.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>