A neuron has a very distinctive form – a bush of dendrites that receive signals, an incredibly long axon, which is like a long tail, and “a little dot” between them that is the cell body, housing the genetic headquarters. Every part of the neuron is required for it to transmit messages.
“If anything breaks along any part of the neuron, the cell unplugs from the circuit and no longer functions,” explained Dr. Freeman, who presented this research at the Genetics Society of America’s 54th Annual Drosophila Research Conference in Washington, D.C.
Once the long tail-like axon is damaged, it shrivels away, basically self-destructing, and resulting in neurons that no longer operate. This catastrophic damage can happen in several ways: from inflammation, a neurodegenerative disease, a metabolic disorder such as diabetes, toxin exposure, or tumor growth. Such axon loss is thought to be a primary factor that leads to functional loss in patients with neurological disorders – it is equivalent to going into an electrical circuit and randomly cutting wires.
The study of axon destruction in response to damage goes back to British neurophysiologist Augustus Waller, who in 1850 described how an axon separated from the cell body and cut off from its nutrient supply breaks apart and is dismantled by scavenger cells. “The idea that this process, called Wallerian degeneration, was a passive wasting away of the axon held for 150 years,” Dr. Freeman said.
Then in the late 1980s, researchers discovered a mutation in the mouse, called Wlds, which enables a damaged axon to survive for weeks after injury. “That fundamentally changed how we think about an axon. Under certain circumstances, axons can survive for a much longer time than we have given them credit for,” Dr. Freeman explained.
Freeman’s laboratory speculated that if axon self-destruction is an active process, then there should be genes in the fly genome whose normal function is to destroy cut axons. They decided if they could break those genes responsible for axon destruction, then the axons shouldn’t fall apart. To identify those genes, they performed a labor-intensive screen, randomly breaking genes in the fly genome and looking for those that when broken blocked axon destruction after injury.
This approach led to the identification of one gene, called dSarm, whose normal function is to promote the destruction of the axon after injury. “We got beautiful protection of axons when we knocked out this molecule,” Dr. Freeman said. Mice and humans have forms of this gene too, and Freeman and colleagues have shown its functions in a similar way in mice. The preservation of these signaling mechanisms from flies to humans is a sign of evolutionary retention and argues for its importance.
To get closer to applying the axon death gene to the study of disease, the researchers crossed the mouse version of the Sarm mutation into a mouse model that has a type of familial ALS, which is also in humans. Although the mice still lost weight and had difficulty with a mobility test, they lived about 10 days longer than their brethren without the Sarm mutation, and at least half of their motor neurons remained intact. “Since not all the motor neurons are needed,” Dr. Freeman said, “even with a 50 percent reduction a patient could feel very close to normal. It would be life-changing for the patient, so it’s a step in the right direction.”
“We used Wallerian degeneration as a model for axon degeneration. We’ve identified a signal pathway whose normal function is to promote axon destruction after injury, and hope to build on this research to better understand the role of axon death in neurodegenerative diseases,” Dr. Freeman summed up.
ABOUT THE GSA DROSOPHILA RESEARCH CONFERENCE: Nearly 1,500 researchers attend the annual GSA Drosophila Research Conference to share the latest research using the fruit fly Drosophila melanogaster and other insect species. Many of findings from these model organisms have broad application for the study of human genetic traits and diseases. For more information about the conference, see http://www.dros-conf.org/2013/
ABOUT GSA: Founded in 1931, the Genetics Society of America (GSA) is the professional membership organization for scientific researchers, educators, bioengineers, bioinformaticians and others interested in the field of genetics. Its nearly 5,000 members work to advance knowledge in the basic mechanisms of inheritance, from the molecular to the population level. The GSA is dedicated to promoting research in genetics and to facilitating communication among geneticists worldwide through its conferences, including the biennial conference on Model Organisms to Human Biology, an interdisciplinary meeting on current and cutting edge topics in genetics research, as well as annual and biennial meetings that focus on the genetics of particular organisms, including C. elegans, Drosophila, fungi, mice, yeast, and zebrafish. GSA publishes GENETICS, a leading journal in the field and a new online, open-access publication, G3: Genes|Genomes|Genetics. For more information about GSA, please visit www.genetics-gsa.org. Also follow GSA on Facebook at facebook.com/GeneticsGSA and on Twitter @GeneticsGSA.
Phyllis Edelman | Newswise
Antimicrobial substances identified in Komodo dragon blood
23.02.2017 | American Chemical Society
New Mechanisms of Gene Inactivation may prevent Aging and Cancer
23.02.2017 | Leibniz-Institut für Alternsforschung - Fritz-Lipmann-Institut e.V. (FLI)
In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport
Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...
The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.
The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...
Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...
Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".
Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...
13.02.2017 | Event News
10.02.2017 | Event News
09.02.2017 | Event News
23.02.2017 | Physics and Astronomy
23.02.2017 | Earth Sciences
23.02.2017 | Life Sciences