Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

A Fluorescent Test System for Hunting Deadly Bacteria

15.04.2011
A fluorescent test system

You can’t see them, or smell them or taste them.

They can be in our water and in our food, multiplying so rapidly that conventional testing methods for detecting pathogens such as E.coli, Salmonella and Listeria come too late for the tens of thousands of Canadians who suffer the ill effects of these deadly bacteria.

Biochemist Yingfu Li and his research team have developed a simple test that can swiftly and accurately identify specific pathogens using a system that will ‘hunt’ for bacteria, identifying their harmful presence before they have a chance to contaminate our food and water.

Like any living thing, bacteria have their own spoor, leaving behind DNA trails of bacterial ‘droppings’. Li tracks these metabolic by-products with molecular beacons – little lighthouses on a molecular scale that actually light up when they detect the DNA sequence left behind.

Li created a DNAzyme sensor that will be able to identify any bacteria, utilizing a method that doesn’t require the steps and specialized equipment typically used to identify whether or not harmful bacteria are present.

“Current methods of foodborne bacterial detection take time. The five days it takes to detect listeria, for example, can translate into an outbreak that costs lives. We have developed a universal test that uses less complex procedures but still generates precise and accurate results,” says Li, a Canada Research Chair in Directed Evolution of Nucleic Acids.

Li’s fluorescent test system was highlighted in Angewandte Chemie International Edition, a prestigious weekly chemistry journal that ranks among the best for the original research it publishes. Li’s paper, co-authored with lab members Monsur Ali, Sergio Aguirre and Hadeer Lazim, was designated a ‘hot paper’ by Angewandte’s editors for its “importance in a rapidly evolving field of current interest”.

“McMaster researchers are known for their ability to provide solutions to problems that impact the public’s well-being. The test that Professor Li has developed will help safeguard the health of Canadians, and supply industry with a reliable means to bring safe food products to consumers and reduce their time to market,” said Mo Elbestawi, vice-president, research and international affairs.

Li’s research was funded by the Natural Sciences and Engineering Research Council (NSERC) and the Sentinel Bioactive Paper Network.

For a photo and full copy of the paper, visit:
http://onlinelibrary.wiley.com/doi/10.1002/anie.201100477/full
For more information, please contact:
Professor Yingfu Li
Associate Professor, Dept. of Biochemistry & Biomedical Sciences and
Canada Research Chair in Directed Evolution of Nucleic Acids
905-525-9140 extension 22462
or liying@mcmaster.ca
Veronica McGuire
Media Relations
Faculty of Health Sciences
McMaster University
905-525-9140, ext. 22169
vmcguir@mcmaster.ca

Veronica McGuire | Newswise Science News
Further information:
http://www.mcmaster.ca

More articles from Life Sciences:

nachricht Nanoparticle Exposure Can Awaken Dormant Viruses in the Lungs
16.01.2017 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt

nachricht Cholera bacteria infect more effectively with a simple twist of shape
13.01.2017 | Princeton University

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Designing Architecture with Solar Building Envelopes

Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.

As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...

Im Focus: How to inflate a hardened concrete shell with a weight of 80 t

At TU Wien, an alternative for resource intensive formwork for the construction of concrete domes was developed. It is now used in a test dome for the Austrian Federal Railways Infrastructure (ÖBB Infrastruktur).

Concrete shells are efficient structures, but not very resource efficient. The formwork for the construction of concrete domes alone requires a high amount of...

Im Focus: Bacterial Pac Man molecule snaps at sugar

Many pathogens use certain sugar compounds from their host to help conceal themselves against the immune system. Scientists at the University of Bonn have now, in cooperation with researchers at the University of York in the United Kingdom, analyzed the dynamics of a bacterial molecule that is involved in this process. They demonstrate that the protein grabs onto the sugar molecule with a Pac Man-like chewing motion and holds it until it can be used. Their results could help design therapeutics that could make the protein poorer at grabbing and holding and hence compromise the pathogen in the host. The study has now been published in “Biophysical Journal”.

The cells of the mouth, nose and intestinal mucosa produce large quantities of a chemical called sialic acid. Many bacteria possess a special transport system...

Im Focus: Newly proposed reference datasets improve weather satellite data quality

UMD, NOAA collaboration demonstrates suitability of in-orbit datasets for weather satellite calibration

"Traffic and weather, together on the hour!" blasts your local radio station, while your smartphone knows the weather halfway across the world. A network of...

Im Focus: Repairing defects in fiber-reinforced plastics more efficiently

Fiber-reinforced plastics (FRP) are frequently used in the aeronautic and automobile industry. However, the repair of workpieces made of these composite materials is often less profitable than exchanging the part. In order to increase the lifetime of FRP parts and to make them more eco-efficient, the Laser Zentrum Hannover e.V. (LZH) and the Apodius GmbH want to combine a new measuring device for fiber layer orientation with an innovative laser-based repair process.

Defects in FRP pieces may be production or operation-related. Whether or not repair is cost-effective depends on the geometry of the defective area, the tools...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

Nothing will happen without batteries making it happen!

05.01.2017 | Event News

 
Latest News

Multiregional brain on a chip

16.01.2017 | Power and Electrical Engineering

New technology enables 5-D imaging in live animals, humans

16.01.2017 | Information Technology

Researchers develop environmentally friendly soy air filter

16.01.2017 | Power and Electrical Engineering

VideoLinks
B2B-VideoLinks
More VideoLinks >>>