Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

3-D microgels 'on-demand' offer new potential for cell research

27.02.2014

Stars, diamonds, circles.

Rather than your average bowl of Lucky Charms, these are three-dimensional cell cultures generated by an exciting new digital microfluidics platform, the results of which have been published in Nature Communications this week by researchers at the University of Toronto. The tool, which can be used to study cells in cost-efficient, three-dimensional microgels, may hold the key to personalized medicine applications in the future.

"We already know that the microenvironment can greatly influence cell fate," says Irwin A. Eydelnant, recent doctoral graduate from IBBME and first author of the publication. "The important part of this study is that we've developed a tool that will allow us to investigate the sensitivity of cells to their 3D environment."

"Everyone wants to do three-dimensional (3D) cell culture," explains Aaron Wheeler, Professor and Canada Research Chair in Bioanalytical Chemistry at the Institute of Biomaterials & Biomedical Engineering (IBBME), the Department of Chemistry, and the Donnelly Centre for Cellular and Biomolecular Research (DCCBR) at the University of Toronto. "Cells grown in this manner share much more in common with living systems than the standard two-dimensional (2D) cell culture format," says Wheeler, corresponding author of the study.

But more naturalistic, 3D cell cultures are a challenge to grow. "The reagents are expensive, the materials are inconvenient for automation, and 3D matrices break down upon repeated handling," explains Wheeler, who was named an Inventor of the Year by the University of Toronto in 2012.

Eydelnant was able to address these difficulties by adapting a digital microfluidics platform first created in the Wheeler lab. Cells, caught up in a hydrogel material, are gently flowed across a small field that, on a screen, looks much like a tiny chessboard. The cells are strategically manipulated by a small electric field across a cutout shape on the top plate of the system, made from indium in oxide, and become fixed.

"When we grew kidney cells in these microgels, the cultures formed hollow sphere structures resembling primitive kidneys within four or five days," Eydelnant claims.

The tool allows a great deal of flexibility in terms of the number of different kinds of cells that can be incorporated into the shapes, as well as the shapes and size of the microenvironments: whimsical, like the stars, diamond and circles of Lucky Charms, or designed to mimic living 3D niches, offering researchers a glimpse into how these factors all affect cell fate decisions.

What's more, according to Eydelnant, the platform permits researchers to run, "32 experiments at the same time, automatically, and all on something the size of a credit card."

"[This new] system allows for hands-free assembly of sub-microlitre, three-dimensional microgels. Each gel is individually addressable, fluid exchange is gentler than macro-scale alternatives, and reagent use is reduced more than 100-fold," Wheeler says.

"We believe that this new tool will make 3D cell culture a more attractive and accessible format for cell biology research," he adds.

Although the researchers can foresee numerous possible applications for this platform, the team is "particularly excited" about its potential for personalized medicine.

Wheeler argues, "We may be able to collect small tissue samples from patients, distribute them into 3D gels on digital microfluidic devices, and screen for conditions to identify individually tailored therapies. This is in the 'dream' stages for now, but we think the methods described here will be useful for these types of applications in the future."

Erin Vollick | EurekAlert!
Further information:
http://www.utoronto.ca

Further reports about: Biomedical Biomolecular Cells Cellular Engineering Inventor culture microfluidics

More articles from Life Sciences:

nachricht Immune Defense Without Collateral Damage
23.01.2017 | Universität Basel

nachricht The interactome of infected neural cells reveals new therapeutic targets for Zika
23.01.2017 | D'Or Institute for Research and Education

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Quantum optical sensor for the first time tested in space – with a laser system from Berlin

For the first time ever, a cloud of ultra-cold atoms has been successfully created in space on board of a sounding rocket. The MAIUS mission demonstrates that quantum optical sensors can be operated even in harsh environments like space – a prerequi-site for finding answers to the most challenging questions of fundamental physics and an important innovation driver for everyday applications.

According to Albert Einstein's Equivalence Principle, all bodies are accelerated at the same rate by the Earth's gravity, regardless of their properties. This...

Im Focus: Traffic jam in empty space

New success for Konstanz physicists in studying the quantum vacuum

An important step towards a completely new experimental access to quantum physics has been made at University of Konstanz. The team of scientists headed by...

Im Focus: How gut bacteria can make us ill

HZI researchers decipher infection mechanisms of Yersinia and immune responses of the host

Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...

Im Focus: Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.

While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...

Im Focus: Studying fundamental particles in materials

Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales

Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Sustainable Water use in Agriculture in Eastern Europe and Central Asia

19.01.2017 | Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

 
Latest News

Tracking movement of immune cells identifies key first steps in inflammatory arthritis

23.01.2017 | Health and Medicine

Electrocatalysis can advance green transition

23.01.2017 | Physics and Astronomy

New technology for mass-production of complex molded composite components

23.01.2017 | Process Engineering

VideoLinks
B2B-VideoLinks
More VideoLinks >>>