Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

3-D long-term bone marrow culture to analyze stromal cell biological function

02.11.2011
Stromal cells, as distinct from hematopoietic cells, are an essential component of the bone marrow microenvironment and are necessary for the long-term maintenance of hematopoietic stem cells (HSCs) in vitro.

Previous studies have shown that stromal cells regulate the proliferation and differentiation of HSCs through the production of diffusible hematopoietic regulatory factors and extracellular matrix, and through physical cell-cell interactions involving adhesion molecules and gap junction-mediated cell communication.

However, the ability of stromal cells to support the expansion of HSCs and to maintain their self-renewal potential has generally been investigated in long-term, two-dimensional (2D) bone marrow culture systems (BMCS), and most of the reports have shown a decline in HSCs within 4 weeks in culture.

In work published in the November 2011 issue of Experimental Biology and Medicine, Hirabayashi and co-investigators from Nihon University School of Medicine, Osaka Prefecture University and the Norwegian University of Science and Technology have developed a new three-dimensional (3D) BMCS. As stated by co-author Isao Tsuboi, "2D BMCS can't maintain HSCs for a long time, which does not enable us to analyze stromal-cell function. Therefore, we developed a new 3D BMCS and succeeded to maintain HSCs for much longer time."

This new 3D BMCS is based on unique particles. As explained by co-author Yukio Hirabayashi, "The polymer particle with grafted epoxy-polymer-chains is most important in our 3D BMCS. We selected the particle most suitable for cell culture from more than 20 types of particles with various grafted polymer chain lengths and its surface density, the composition of base polymer network and graft polymer chain. We named this particle G-02". Furthermore, co-author Tomonori Harada adds "Several kinds of cell lines, other than murine fibroblast cell line (MS-5 cell), such as an epidermal cell line (HeLa cell), osteoblast cell line (MC3T3E1 cell) and chondrocyte cell line (ch-8 cell), can adhere easily on the G-02 particle and proliferate rapidly on its surface. This advantage of the G-02 particle enables us to develop 3D BMCS, which can also be applied to other 3D organ cultures of central nerve system, heart and liver."

CD34 is well known to be a surface marker for human primitive hematopoietic progenitor cells, however, CD34+ cells can also differentiate into stromal cells. When CD34+ cells are co-cultured with human stromal cells, it is complex to clarify the biological function of the preestablished stromal cell layer. Prof. Shin Aizawa, group leader and pioneer in the study of stromal cell, says "In this study, we used a murine stromal cell line (MS-5) instead of human stromal cells to exclude the effect of CD34+ derived stromal cells. This co-culture system makes it possible to distinguish the function of MS-5 stromal-cell layer from that of CD34+ derived stromal cells. Now our group is studying gene-expression levels of various cytokines in stromal cells using specific primers and probes for the mouse and human".

Steven R. Goodman, Ph.D. Editor-in-Chief of Experimental Biology and Medicine said "this 3-dimensional bone marrow culture system, developed by Hirabayashi and coworkers, should be an outstanding tool for the study stromal cell function".

Experimental Biology and Medicine is a journal dedicated to the publication of multidisciplinary and interdisciplinary research in the biomedical sciences. The journal was first established in 1903. Experimental Biology and Medicine is the journal of the Society of Experimental Biology and Medicine. To learn about the benefits of society membership visit www.sebm.org. If you are interested in publishing in the journal please visit http://ebm.rsmjournals.com.

Dr. Tomonori Harada | EurekAlert!
Further information:
http://www.sebm.org

More articles from Life Sciences:

nachricht How brains surrender to sleep
23.06.2017 | IMP - Forschungsinstitut für Molekulare Pathologie GmbH

nachricht A new technique isolates neuronal activity during memory consolidation
22.06.2017 | Spanish National Research Council (CSIC)

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Can we see monkeys from space? Emerging technologies to map biodiversity

An international team of scientists has proposed a new multi-disciplinary approach in which an array of new technologies will allow us to map biodiversity and the risks that wildlife is facing at the scale of whole landscapes. The findings are published in Nature Ecology and Evolution. This international research is led by the Kunming Institute of Zoology from China, University of East Anglia, University of Leicester and the Leibniz Institute for Zoo and Wildlife Research.

Using a combination of satellite and ground data, the team proposes that it is now possible to map biodiversity with an accuracy that has not been previously...

Im Focus: Climate satellite: Tracking methane with robust laser technology

Heatwaves in the Arctic, longer periods of vegetation in Europe, severe floods in West Africa – starting in 2021, scientists want to explore the emissions of the greenhouse gas methane with the German-French satellite MERLIN. This is made possible by a new robust laser system of the Fraunhofer Institute for Laser Technology ILT in Aachen, which achieves unprecedented measurement accuracy.

Methane is primarily the result of the decomposition of organic matter. The gas has a 25 times greater warming potential than carbon dioxide, but is not as...

Im Focus: How protons move through a fuel cell

Hydrogen is regarded as the energy source of the future: It is produced with solar power and can be used to generate heat and electricity in fuel cells. Empa researchers have now succeeded in decoding the movement of hydrogen ions in crystals – a key step towards more efficient energy conversion in the hydrogen industry of tomorrow.

As charge carriers, electrons and ions play the leading role in electrochemical energy storage devices and converters such as batteries and fuel cells. Proton...

Im Focus: A unique data centre for cosmological simulations

Scientists from the Excellence Cluster Universe at the Ludwig-Maximilians-Universität Munich have establised "Cosmowebportal", a unique data centre for cosmological simulations located at the Leibniz Supercomputing Centre (LRZ) of the Bavarian Academy of Sciences. The complete results of a series of large hydrodynamical cosmological simulations are available, with data volumes typically exceeding several hundred terabytes. Scientists worldwide can interactively explore these complex simulations via a web interface and directly access the results.

With current telescopes, scientists can observe our Universe’s galaxies and galaxy clusters and their distribution along an invisible cosmic web. From the...

Im Focus: Scientists develop molecular thermometer for contactless measurement using infrared light

Temperature measurements possible even on the smallest scale / Molecular ruby for use in material sciences, biology, and medicine

Chemists at Johannes Gutenberg University Mainz (JGU) in cooperation with researchers of the German Federal Institute for Materials Research and Testing (BAM)...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Plants are networkers

19.06.2017 | Event News

Digital Survival Training for Executives

13.06.2017 | Event News

Global Learning Council Summit 2017

13.06.2017 | Event News

 
Latest News

Quantum thermometer or optical refrigerator?

23.06.2017 | Physics and Astronomy

A 100-year-old physics problem has been solved at EPFL

23.06.2017 | Physics and Astronomy

Equipping form with function

23.06.2017 | Information Technology

VideoLinks
B2B-VideoLinks
More VideoLinks >>>