Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Collaboration at EMSL produces innovative mass spectrometer

02.06.2003


The future of proteomics is in good hands with one of the most powerful and versatile mass spectrometers being developed by scientists and engineers from the William R. Wiley Environmental Molecular Sciences Laboratory.



The high-throughput Fourier transform ion cyclotron resonance (FTICR) mass spectrometer and automated liquid chromatography (LC) system is a breakthrough in mass spectrometry capable of improving the understanding of protein production, function and interactions at the cellular level and beyond.

Proteomics is the study of proteins in the human genome that are made and change within a cell over time as the cells respond to disease or changes in their surrounding environment.


"The success of the high- throughput FTICR mass spectrometer and the automation system heralds the next generation of proteomics research," said Harold Udseth, technical leader of EMSL’s High Performance Mass Spectrometry Facility.

Mass spectrometers are used to weigh atoms and molecules. This can be done with a precision of one part in five million, which, when applied to the human cell, will enable scientists to learn a great deal about how the cell works. "And once scientists fully understand how human cells work, they can begin to work on methodically solving problems involving things that go wrong in a cell such as cancer," Udseth said.

"The signature of the newest, high-throughput FTICR is the high resolution and high mass measurement accuracy of the system," Udseth continued. "It does better than other mass spectrometers available in measuring the mass of peptides (small structural units obtained by cutting proteins into pieces). It offers such sensitivity and precision that scientists can detect hundreds of thousands of peptide species in a single analysis." Identifying peptides is an important part of proteomics research to determine the role that proteins play in cells and living systems. Researchers expect that more than a million peptides are available in the proteins expressed within human cells.

The mass spectrometer project took nine months of concerted effort by a diverse team composed of scientists from the High Performance Mass Spectrometry Facility and engineers at the EMSL Instrument Development Laboratory (IDL), both of whom brought diverse talent and experience to the table. "It’s essential to get the right people in the right place, people who have a desire to work together for a common goal," Udseth said. "In this case, everyone was inspired by the work."

The project required the replacement of the instrument’s commercial ion manipulation optics with EMSL’s patented ion funnel technology, as well as the design, development and construction of an automated LC system that incorporates a commercial autosampler and PNNL’s high-pressure LC technology. Software written by researchers at EMSL is used to control the LC system and integrate it into a single operating system.

"We worked closely with Harold and his staff to understand and provide technical support to overcome the unique challenges of developing this cutting-edge research equipment," said Gordon Anderson, manager of the IDL. The IDL is an integral part of EMSL and includes staff with expertise in electronic circuit design and fabrication as well as software development. "Finding that common, underlying language between scientists and engineers paved the way to a satisfying collaboration," Anderson said.


A multidisciplinary team composed of scientists and engineers was key to the successful development of the cutting-edge, high-throughput Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. The research tool significantly accelerates proteome analysis and provides accuracy and depth never before reached in proteome studies. This ability greatly enhances the understanding of protein production, function and interactions at the cellular level.

Greg Koller | DOE/PNNL
Further information:
http://www.emsl.pnl.gov
http://www.emsl.pnl.gov:2080/docs/idl/home.html
http://www.pnl.gov/breakthroughs/winter-spring03/special_report3.stm#fticr

More articles from Interdisciplinary Research:

nachricht Bergamotene - alluring and lethal for Manduca sexta
21.04.2017 | Max-Planck-Institut für chemische Ökologie

nachricht How to color a lizard: From biology to mathematics
13.04.2017 | Université de Genève

All articles from Interdisciplinary Research >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Carbon Nanotubes Turn Electrical Current into Light-emitting Quasi-particles

Strong light-matter coupling in these semiconducting tubes may hold the key to electrically pumped lasers

Light-matter quasi-particles can be generated electrically in semiconducting carbon nanotubes. Material scientists and physicists from Heidelberg University...

Im Focus: Flexible proximity sensor creates smart surfaces

Fraunhofer IPA has developed a proximity sensor made from silicone and carbon nanotubes (CNT) which detects objects and determines their position. The materials and printing process used mean that the sensor is extremely flexible, economical and can be used for large surfaces. Industry and research partners can use and further develop this innovation straight away.

At first glance, the proximity sensor appears to be nothing special: a thin, elastic layer of silicone onto which black square surfaces are printed, but these...

Im Focus: 3-D scanning with water

3-D shape acquisition using water displacement as the shape sensor for the reconstruction of complex objects

A global team of computer scientists and engineers have developed an innovative technique that more completely reconstructs challenging 3D objects. An ancient...

Im Focus: Manipulating Electron Spins Without Loss of Information

Physicists have developed a new technique that uses electrical voltages to control the electron spin on a chip. The newly-developed method provides protection from spin decay, meaning that the contained information can be maintained and transmitted over comparatively large distances, as has been demonstrated by a team from the University of Basel’s Department of Physics and the Swiss Nanoscience Institute. The results have been published in Physical Review X.

For several years, researchers have been trying to use the spin of an electron to store and transmit information. The spin of each electron is always coupled...

Im Focus: The proton precisely weighted

What is the mass of a proton? Scientists from Germany and Japan successfully did an important step towards the most exact knowledge of this fundamental constant. By means of precision measurements on a single proton, they could improve the precision by a factor of three and also correct the existing value.

To determine the mass of a single proton still more accurate – a group of physicists led by Klaus Blaum and Sven Sturm of the Max Planck Institute for Nuclear...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Closing the Sustainability Circle: Protection of Food with Biobased Materials

21.07.2017 | Event News

»We are bringing Additive Manufacturing to SMEs«

19.07.2017 | Event News

The technology with a feel for feelings

12.07.2017 | Event News

 
Latest News

NASA mission surfs through waves in space to understand space weather

25.07.2017 | Physics and Astronomy

Strength of tectonic plates may explain shape of the Tibetan Plateau, study finds

25.07.2017 | Earth Sciences

The dense vessel network regulates formation of thrombocytes in the bone marrow

25.07.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>