Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Novel Computed Imaging Technique Uses Blurry Images To Enhance View

22.01.2007
Researchers at the University of Illinois at Urbana-Champaign have developed a novel computational image-forming technique for optical microscopy that can produce crisp, three-dimensional images from blurry, out-of-focus data.

Called Interferometric Synthetic Aperture Microscopy, ISAM can do for optical microscopy what magnetic resonance imaging did for nuclear magnetic resonance, and what computed tomography did for X-ray imaging, the scientists say.

"ISAM can perform high-speed, micron-scale, cross-sectional imaging without the need for time-consuming processing, sectioning and staining of resected tissue," said Stephen Boppart, a professor of electrical and computer engineering, of bioengineering, and of medicine at the U. of I., and corresponding author of a paper accepted for publication in the journal Nature Physics, and posted on its Web site.

Developed by postdoctoral research associate and lead author Tyler Ralston, research scientist Daniel Marks, electrical and computer engineering professor P. Scott Carney, and Boppart, the imaging technique utilizes a broad-spectrum light source and a spectral interferometer to obtain high-resolution, reconstructed images from the optical signals based on an understanding of the physics of light-scattering within the sample.

"ISAM has the potential to broadly impact real-time, three-dimensional microscopy and analysis in the fields of cell and tumor biology, as well as in clinical diagnosis where imaging is preferable to biopsy," said Boppart, who is also a physician and founding director of the Mills Breast Cancer Institute at Carle Foundation Hospital in Urbana, Ill.

While other methods of three-dimensional optical microscopy require the instrument's focal plane to be scanned through the region of interest, ISAM works by utilizing light from the out-of-focus image planes, Ralston said. "Although most of the image planes are blurry, ISAM descrambles the light to produce a fully focused, three-dimensional image."

ISAM effectively extends the region of the image that is in focus, using information that was discarded in the past.

"We have demonstrated that the discarded information can be computationally reconstructed to quickly create the desired image," Marks said. "We are now applying the technique to various microscopy methods used in biological imaging."

In their paper, the researchers demonstrate the usefulness of computed image reconstruction on both phantom tissue and on excised human breast-tumor tissue.

"ISAM can assist doctors by providing faster diagnostic information, and by facilitating the further development of image-guided surgery," Boppart said. "Using ISAM, it may be possible to perform micron-scale imaging over large volumes of tissue rather than resecting large volumes of tissue."

The versatile imaging technique can be applied to existing hardware with only minor modifications.

In addition to previously mentioned affiliations, Boppart, Carney, Marks and Ralston hold positions within the department of electrical and computer engineering and are affiliated with the U. of I.'s Beckman Institute for Advanced Science and Technology. Boppart also is affiliated with the university's Micro and Nanotechnology Laboratory and the Institute for Genomic Biology; Carney also is affiliated with the university's Coordinated Science Laboratory.

The National Institutes of Health, National Science Foundation, and the Beckman Institute funded the work.

mes E. Kloeppel | University of Illinois
Further information:
http://www.uiuc.edu

More articles from Information Technology:

nachricht New technology enables 5-D imaging in live animals, humans
16.01.2017 | University of Southern California

nachricht Fraunhofer FIT announces CloudTeams collaborative software development platform – join it for free
10.01.2017 | Fraunhofer-Institut für Angewandte Informationstechnik FIT

All articles from Information Technology >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Designing Architecture with Solar Building Envelopes

Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.

As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...

Im Focus: How to inflate a hardened concrete shell with a weight of 80 t

At TU Wien, an alternative for resource intensive formwork for the construction of concrete domes was developed. It is now used in a test dome for the Austrian Federal Railways Infrastructure (ÖBB Infrastruktur).

Concrete shells are efficient structures, but not very resource efficient. The formwork for the construction of concrete domes alone requires a high amount of...

Im Focus: Bacterial Pac Man molecule snaps at sugar

Many pathogens use certain sugar compounds from their host to help conceal themselves against the immune system. Scientists at the University of Bonn have now, in cooperation with researchers at the University of York in the United Kingdom, analyzed the dynamics of a bacterial molecule that is involved in this process. They demonstrate that the protein grabs onto the sugar molecule with a Pac Man-like chewing motion and holds it until it can be used. Their results could help design therapeutics that could make the protein poorer at grabbing and holding and hence compromise the pathogen in the host. The study has now been published in “Biophysical Journal”.

The cells of the mouth, nose and intestinal mucosa produce large quantities of a chemical called sialic acid. Many bacteria possess a special transport system...

Im Focus: Newly proposed reference datasets improve weather satellite data quality

UMD, NOAA collaboration demonstrates suitability of in-orbit datasets for weather satellite calibration

"Traffic and weather, together on the hour!" blasts your local radio station, while your smartphone knows the weather halfway across the world. A network of...

Im Focus: Repairing defects in fiber-reinforced plastics more efficiently

Fiber-reinforced plastics (FRP) are frequently used in the aeronautic and automobile industry. However, the repair of workpieces made of these composite materials is often less profitable than exchanging the part. In order to increase the lifetime of FRP parts and to make them more eco-efficient, the Laser Zentrum Hannover e.V. (LZH) and the Apodius GmbH want to combine a new measuring device for fiber layer orientation with an innovative laser-based repair process.

Defects in FRP pieces may be production or operation-related. Whether or not repair is cost-effective depends on the geometry of the defective area, the tools...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

Nothing will happen without batteries making it happen!

05.01.2017 | Event News

 
Latest News

Multiregional brain on a chip

16.01.2017 | Power and Electrical Engineering

New technology enables 5-D imaging in live animals, humans

16.01.2017 | Information Technology

Researchers develop environmentally friendly soy air filter

16.01.2017 | Power and Electrical Engineering

VideoLinks
B2B-VideoLinks
More VideoLinks >>>