Called Interferometric Synthetic Aperture Microscopy, ISAM can do for optical microscopy what magnetic resonance imaging did for nuclear magnetic resonance, and what computed tomography did for X-ray imaging, the scientists say.
"ISAM can perform high-speed, micron-scale, cross-sectional imaging without the need for time-consuming processing, sectioning and staining of resected tissue," said Stephen Boppart, a professor of electrical and computer engineering, of bioengineering, and of medicine at the U. of I., and corresponding author of a paper accepted for publication in the journal Nature Physics, and posted on its Web site.
Developed by postdoctoral research associate and lead author Tyler Ralston, research scientist Daniel Marks, electrical and computer engineering professor P. Scott Carney, and Boppart, the imaging technique utilizes a broad-spectrum light source and a spectral interferometer to obtain high-resolution, reconstructed images from the optical signals based on an understanding of the physics of light-scattering within the sample.
"ISAM has the potential to broadly impact real-time, three-dimensional microscopy and analysis in the fields of cell and tumor biology, as well as in clinical diagnosis where imaging is preferable to biopsy," said Boppart, who is also a physician and founding director of the Mills Breast Cancer Institute at Carle Foundation Hospital in Urbana, Ill.
While other methods of three-dimensional optical microscopy require the instrument's focal plane to be scanned through the region of interest, ISAM works by utilizing light from the out-of-focus image planes, Ralston said. "Although most of the image planes are blurry, ISAM descrambles the light to produce a fully focused, three-dimensional image."
ISAM effectively extends the region of the image that is in focus, using information that was discarded in the past.
"We have demonstrated that the discarded information can be computationally reconstructed to quickly create the desired image," Marks said. "We are now applying the technique to various microscopy methods used in biological imaging."
In their paper, the researchers demonstrate the usefulness of computed image reconstruction on both phantom tissue and on excised human breast-tumor tissue.
"ISAM can assist doctors by providing faster diagnostic information, and by facilitating the further development of image-guided surgery," Boppart said. "Using ISAM, it may be possible to perform micron-scale imaging over large volumes of tissue rather than resecting large volumes of tissue."
The versatile imaging technique can be applied to existing hardware with only minor modifications.
In addition to previously mentioned affiliations, Boppart, Carney, Marks and Ralston hold positions within the department of electrical and computer engineering and are affiliated with the U. of I.'s Beckman Institute for Advanced Science and Technology. Boppart also is affiliated with the university's Micro and Nanotechnology Laboratory and the Institute for Genomic Biology; Carney also is affiliated with the university's Coordinated Science Laboratory.
The National Institutes of Health, National Science Foundation, and the Beckman Institute funded the work.
mes E. Kloeppel | University of Illinois
21.08.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau
AI implications: Engineer's model lays groundwork for machine-learning device
18.08.2017 | Washington University in St. Louis
Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.
As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...
Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.
Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...
For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.
While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...
An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.
The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...
A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.
Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...
16.08.2017 | Event News
04.08.2017 | Event News
26.07.2017 | Event News
22.08.2017 | Life Sciences
22.08.2017 | Life Sciences
22.08.2017 | Life Sciences