Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Embryos exposed in 3-D

08.05.2006


New method can identify what genes do, test drugs’ safety



Utah and Texas researchers combined miniature medical CT scans with high-tech computer methods to produce detailed three-dimensional images of mouse embryos – an efficient new method to test the safety of medicines and learn how mutant genes cause birth defects or cancer.

"Our method provides a fast, high-quality and inexpensive way to visually explore the 3-D internal structure of mouse embryos so scientists can more easily and quickly see the effects of a genetic defect or chemical damage," says Chris Johnson, a distinguished professor of computer science at the University of Utah.


A study reporting development of the new method – known as "microCT-based virtual histology" – was published recently in PLoS Genetics, an online journal of the Public Library of Science.

The study was led by Charles Keller, a pediatric cancer specialist who formerly worked as a postdoctoral fellow in the laboratory of University of Utah geneticist Mario Capecchi. Keller now is an assistant professor at the Children’s Cancer Research Institute at the University of Texas Health Science Center in San Antonio.

University of Utah co-authors of the study are Johnson – who directs the university’s Scientific Computing and Imaging Institute – Capecchi, medical student Mark S. Hansen and several members of Johnson’s institute: computer science undergraduate Thomas Johnson III, research assistant Lindsey Healey and former associate director Greg M. Jones, who now is state science advisor to Utah Gov. Jon Huntsman Jr.

Scientists often use mouse embryos both to learn what genes do and to test the safety of new drugs and household chemicals. By disabling or "knocking out" a gene, researchers can see what goes wrong in the mouse embryo and thus learn the gene’s normal function, or learn how a mutant gene can cause cancer. Mouse embryos also are sensitive to toxicity from chemicals, so new medicines and chemicals are tested on them to see if any defects develop, indicating the safety for humans and their unborn embryos.

But the traditional method of histology – the anatomical study of the microscopic structure of living tissues – has been difficult and time-consuming. Mouse embryos with genetic mutations or damage from toxic chemicals are killed, embedded in wax, sliced into thin sections, then stained and placed on slides for examination under a microscope.

The new, faster and inexpensive method is called "virtual histology" because it uses computer visualization techniques to convert X-ray CT scans of mouse embryos into detailed 3-D images showing both the mouse’s exterior and interior.

Instead of being sliced up physically, mouse embryos are stained with special dyes. Traditional CT scans take a series of X-ray images representing "slices" through the body, and they primarily "see" bone and other hard tissues such as cartilage. In the new microCT virtual histology, the special dyes permeate the skin and other membranes, which are still permeable in an embryo.

"This technique allows us to get at a lot more tissues other than bone, such as internal organs, which [conventional] CT scans can’t pick up," Johnson says.

Johnson and his team wrote a computer algorithm – a problem-solving formula in computer software – to take the CT scan data and automatically distinguish various organs and structures in the mouse embryo. The "virtual rendering" of the CT scan data also includes a virtual light source so the 3-D embryo image includes shadows that make it easier for the human eye to understand and interpret the image.

The embryo images can be made transparent or have cutaways so that internal organs and body parts are visible. And the detail they show is exquisite – revealing features as small as one-tenth the thickness of a human hair.

The idea is to allow geneticists to quickly examine large numbers of embryos, each with a different gene disabled, so that the normal function of many genes can be determined faster than with existing methods.

Keller says the U.S. Food and Drug Administration and Environmental Protection Agency require drug and chemical manufacturers, respectively, to test their new products, but that such tests often are subjective. The new method "allows chemical and drug companies to conduct these studies in a much more quantitative way, improving upon the safety of the products we find in our homes," he adds.

Keller and a colleague have founded a company named Numira Biosciences, which plans to make the virtual histology method available through the sale of kits and imaging services.

The study was published online in the April 28 issue of PLoS Genetics.

Chris Johnson | EurekAlert!
Further information:
http://www.utah.edu

More articles from Information Technology:

nachricht Sensors embedded in sports equipment could provide real-time analytics to your smartphone
16.02.2017 | University of Illinois College of Engineering

nachricht Researchers catch extreme waves with higher-resolution modeling
15.02.2017 | DOE/Lawrence Berkeley National Laboratory

All articles from Information Technology >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Breakthrough with a chain of gold atoms

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

Im Focus: DNA repair: a new letter in the cell alphabet

Results reveal how discoveries may be hidden in scientific “blind spots”

Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...

Im Focus: Dresdner scientists print tomorrow’s world

The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.

The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...

Im Focus: Mimicking nature's cellular architectures via 3-D printing

Research offers new level of control over the structure of 3-D printed materials

Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...

Im Focus: Three Magnetic States for Each Hole

Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".

Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Booth and panel discussion – The Lindau Nobel Laureate Meetings at the AAAS 2017 Annual Meeting

13.02.2017 | Event News

Complex Loading versus Hidden Reserves

10.02.2017 | Event News

International Conference on Crystal Growth in Freiburg

09.02.2017 | Event News

 
Latest News

Biocompatible 3-D tracking system has potential to improve robot-assisted surgery

17.02.2017 | Medical Engineering

Real-time MRI analysis powered by supercomputers

17.02.2017 | Medical Engineering

Antibiotic effective against drug-resistant bacteria in pediatric skin infections

17.02.2017 | Health and Medicine

VideoLinks
B2B-VideoLinks
More VideoLinks >>>