With ability to analyze minuscule amounts of fluid, Rutgers breakthrough could also promote central nervous system and joint research
Rutgers engineers have developed a breakthrough device that can significantly reduce the cost of sophisticated lab tests for medical disorders and diseases, such as HIV, Lyme disease and syphilis.
The new device uses miniaturized channels and valves to replace "benchtop" assays - tests that require large samples of blood or other fluids and expensive chemicals that lab technicians manually mix in trays of tubes or plastic plates with cup-like depressions.
"The main advantage is cost - these assays are done in labs and clinics everywhere," said Mehdi Ghodbane, who earned his doctorate in biomedical engineering at Rutgers and now works in biopharmaceutical research and development at GlaxoSmithKline.
Ghodbane and six Rutgers researchers recently published their results in the Royal Society of Chemistry's journal, Lab on a Chip.
The lab-on-chip device, which employs microfluidics technology, along with making tests more affordable for patients and researchers, opens doors for new research because of its capability to perform complex analyses using 90 percent less sample fluid than needed in conventional tests.
"A great deal of research has been hindered because in many cases one is not able to extract enough fluid," Ghodbane said.
The Rutgers breakthrough also requires one-tenth of the chemicals used in a conventional multiplex immunoassay, which can cost as much as $1500. Additionally, the device automates much of the skilled labor involved in performing tests.
"The results are as sensitive and accurate as the standard benchtop assay,'' said Martin Yarmush, the Paul and Mary Monroe Chair and Distinguished Professor of biomedical engineering at Rutgers and Ghodbane's adviser.
Until now, animal research on central nervous system disorders, such as spinal cord injury and Parkinson's disease, has been limited because researchers could not extract sufficient cerebrospinal fluid to perform conventional assays.
"With our technology, researchers will be able to perform large-scale controlled studies with comparable accuracy to conventional assays," Yarmush said.
The discovery could also lead to more comprehensive research on autoimmune joint diseases such as rheumatoid arthritis through animal studies. As with spinal fluid, the amount of joint fluid, or synovial fluid, researchers are able to collect from lab animals is minuscule.
The Rutgers team has combined several capabilities for the first time in the device they've dubbed "ELISA-on-a-chip" (for enzyme-linked immunosorbent assay). A single device analyzes 32 samples at once and can measure widely varying concentrations of as many as six proteins in a sample.
The researchers are exploring the commercial potential of their technology.
Other members of the research team are Elizabeth Stucky, a doctoral student in the Department of Chemical and Biochemical Engineering, and assistant research professor Tim Maguire, associate research professor Rene Schloss, professor David Shreiber and associate professor Jeffrey Zahn, all in the Department of Biomedical Engineering.
The National Institutes of Health, the National Science Foundation, the New Jersey Commission on Brain Injury Research and Corning, Inc. provided funding for the research.
Carl Blesch | EurekAlert!
Quantum Technology for Advanced Imaging – QUILT
24.04.2018 | Fraunhofer-Institut für Lasertechnik ILT
Paint job transforms walls into sensors, interactive surfaces
24.04.2018 | Carnegie Mellon University
At the Hannover Messe 2018, the Bundesanstalt für Materialforschung und-prüfung (BAM) will show how, in the future, astronauts could produce their own tools or spare parts in zero gravity using 3D printing. This will reduce, weight and transport costs for space missions. Visitors can experience the innovative additive manufacturing process live at the fair.
Powder-based additive manufacturing in zero gravity is the name of the project in which a component is produced by applying metallic powder layers and then...
Physicists at the Laboratory for Attosecond Physics, which is jointly run by Ludwig-Maximilians-Universität and the Max Planck Institute of Quantum Optics, have developed a high-power laser system that generates ultrashort pulses of light covering a large share of the mid-infrared spectrum. The researchers envisage a wide range of applications for the technology – in the early diagnosis of cancer, for instance.
Molecules are the building blocks of life. Like all other organisms, we are made of them. They control our biorhythm, and they can also reflect our state of...
University of Connecticut researchers have created a biodegradable composite made of silk fibers that can be used to repair broken load-bearing bones without the complications sometimes presented by other materials.
Repairing major load-bearing bones such as those in the leg can be a long and uncomfortable process.
Study published in the journal ACS Applied Materials & Interfaces is the outcome of an international effort that included teams from Dresden and Berlin in Germany, and the US.
Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) together with colleagues from the Helmholtz-Zentrum Berlin (HZB) and the University of Virginia...
Novel highly efficient and brilliant gamma-ray source: Based on model calculations, physicists of the Max PIanck Institute for Nuclear Physics in Heidelberg propose a novel method for an efficient high-brilliance gamma-ray source. A giant collimated gamma-ray pulse is generated from the interaction of a dense ultra-relativistic electron beam with a thin solid conductor. Energetic gamma-rays are copiously produced as the electron beam splits into filaments while propagating across the conductor. The resulting gamma-ray energy and flux enable novel experiments in nuclear and fundamental physics.
The typical wavelength of light interacting with an object of the microcosm scales with the size of this object. For atoms, this ranges from visible light to...
13.04.2018 | Event News
12.04.2018 | Event News
09.04.2018 | Event News
24.04.2018 | Information Technology
24.04.2018 | Earth Sciences
24.04.2018 | Life Sciences