Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

In subglacial lake, surprising life goes on

05.07.2013
Rogers team identifies species in most inhospitable realm

Lake Vostok, buried under a glacier in Antarctica, is so dark, deep and cold that scientists had considered it a possible model for other planets, a place where nothing could live.

However, work by Dr. Scott Rogers, a Bowling Green State University professor of biological sciences, and his colleagues has revealed a surprising variety of life forms living and reproducing in this most extreme of environments. A paper published June 26 in PLOS ONE (Public Library of Science - http://dx.plos.org/10.1371/journal.pone.0067221) details the thousands of species they identified through DNA and RNA sequencing.

"The bounds on what is habitable and what is not are changing," Rogers said.

This is the fourth article the group has published about its Lake Vostok investigations. The team included Dr. Paul Morris, biology, who with Scott and doctoral student Yury Shtarkman conducted most of the genetic analyses; former doctoral students Zeynep Koçer, now with the Department of Infectious Diseases, Division of Virology, at St. Jude's Research Hospital in Memphis, performed most of the laboratory work; Ram Veerapaneni, now at BGSU Firelands, Tom D'Elia, now at Indian River State College in Florida, and undergraduate student Robyn Edgar, computer science.

Their work was supported by several grants, including two from the National Science Foundation, one from U.S. Department of Agriculture and one from the BGSU Faculty Research Committee. Together, the amount dedicated to the project was more than $250,000.

When thinking about Lake Vostok, you have to think big. The fourth-deepest lake on Earth, it is also the largest of the 400-some subglacial lakes known in Antarctica. The ice that has covered it for the past 15 million years is now more than two miles deep, creating tremendous pressure in the lake. Few nutrients are available. The lake lies far below sea level in a depression that formed 60 million years ago when the continental plates shifted and cracked. The weather there is so harsh and unpredictable that scientists visiting must have special gear and take survival training.

Not only had most scientists believed Lake Vostok completely inhospitable to life, some thought it might even be sterile.

Far from it, Rogers found. Working with core sections removed from the deep layer of ice that accreted from lake water that froze onto the bottom of the glacier where it meets the lake, Rogers examined ice as clear as diamonds that formed in the great pressure and relatively warm temperatures found at that depth. The team sampled cores from two areas of the lake, the southern main basin and near an embayment on the southwestern end of the lake.

"We found much more complexity than anyone thought," Rogers said. "It really shows the tenacity of life, and how organisms can survive in places where a couple dozen years ago we thought nothing could survive."

By sequencing the DNA and RNA from the accretion ice samples, the team identified thousands of bacteria, including some that are commonly found in the digestive systems of fish, crustaceans and annelid worms, in addition to fungi and two species of archaea, or single-celled organisms that tend to live in extreme environments. Other species they identified are associated with habitats of lake or ocean sediments. Psychrophiles, or organisms that live in extreme cold, were found, along with heat-loving thermophiles, which suggests the presence of hydrothermal vents deep in the lake. Rogers said the presence of marine and freshwater species supports the hypothesis that the lake once was connected to the ocean, and that the freshwater was deposited in the lake by the overriding glacier.

The largest number of species overall was found in the area near the embayment, including many that are common to freshwater environments, as well as marine species, psychrophiles and thermophiles. Numerous others were found that remain unidentified. The embayment appears to contain much of the biological activity in the lake.

"Many of the species we sequenced are what we would expect to find in a lake," Rogers said. "Most of the organisms appear to be aquatic (freshwater), and many are species that usually live in ocean or lake sediments."

For Shtarkman, who came to BGSU from St. Petersburg, Russia, the project has proven so engrossing that he foresees a possible lifetime of study around it. "It's a very challenging project and the more you study, the more you want to know," he said. "Every day you are discovering something new and that leads to more questions to be answered. In studying the environmental DNA and RNA, we ask how similar are these sequences to those of sequences from organisms already identified in national databases. We are tracing the evolution and the ecology of the lake itself.

Before 35 million years ago, Antarctica had a temperate climate and was inhabited by a diverse assemblage of plants and animals. About 34 million years ago, Rogers said, a "huge drop in temperature occurred" and ice covered the lake, when it was probably still connected to the Southern Ocean. This lowered sea level by about 300 feet, which could have cut off Lake Vostok from the ocean. The ice cover was intermittent until a second big plunge in temperature took place 14 million years ago, and sea level dropped even farther.

As the ice crept across the lake, it plunged the lake into total darkness and isolated it from the atmosphere, and led to increasing pressure in the lake from the weight of the glacier. While many species probably disappeared from the lake, many seem to have survived, as indicated by Rogers' results.

Rogers's group had worked for several years on identifying and studying organisms in the Vostok accretion ice using a procedure involving culturing colonies of bacteria and fungi, but the process was slow, especially for graduate students who needed results for their theses.

"We started thinking of doing it a different way," Rogers said.

Instead of culturing living organisms, they concentrated on sequencing DNA and RNA in the ice. These methods, called metagenomics and metatranscriptomics, produced thousands of sequences at a time that were then analyzed using computers — procedures referred to collectively as "Big Data" methods. In contrast, it usually took years to generate enough cultured organisms for a few dozen sequences.

The problem changed from having too few sequences to having too many sequences to analyze, Rogers said. After two years of computer analysis, the final results showed that Lake Vostok contains a diverse set of microbes, as well as some multicellular organisms.

Long before he began using metagenomics and metatranscriptomics to study the ice, Rogers and his team had developed a method to ensure purity. Sections of core ice were immersed in a sodium hypochlorite (bleach) solution, then rinsed three times with sterile water, removing an outer layer. Under strict sterile conditions, the remaining core ice was then melted, filtered and refrozen.

"Using this method, we can assure its reliability almost to 100 percent," Rogers said.

Eventually, the process rendered pellets of nucleic acids containing both DNA and RNA, able to be sequenced.

Rogers said the team erred strongly on the conservative side in reporting its results, including only those sequences of which it could be absolutely certain were from the accretion ice, but there are a multitude of others he feels are probably from the lake, opening the door to additional investigation.

The DNA sequences they produced have been deposited in the National Center for Biotechnology GenBank database, and will be available to other researchers for further study.

Jen Sobolewski | EurekAlert!
Further information:
http://www.bgsu.edu

More articles from Earth Sciences:

nachricht Sediment from Himalayas may have made 2004 Indian Ocean earthquake more severe
26.05.2017 | Oregon State University

nachricht Devils Hole: Ancient Traces of Climate History
24.05.2017 | Universität Innsbruck

All articles from Earth Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Can the immune system be boosted against Staphylococcus aureus by delivery of messenger RNA?

Staphylococcus aureus is a feared pathogen (MRSA, multi-resistant S. aureus) due to frequent resistances against many antibiotics, especially in hospital infections. Researchers at the Paul-Ehrlich-Institut have identified immunological processes that prevent a successful immune response directed against the pathogenic agent. The delivery of bacterial proteins with RNA adjuvant or messenger RNA (mRNA) into immune cells allows the re-direction of the immune response towards an active defense against S. aureus. This could be of significant importance for the development of an effective vaccine. PLOS Pathogens has published these research results online on 25 May 2017.

Staphylococcus aureus (S. aureus) is a bacterium that colonizes by far more than half of the skin and the mucosa of adults, usually without causing infections....

Im Focus: A quantum walk of photons

Physicists from the University of Würzburg are capable of generating identical looking single light particles at the push of a button. Two new studies now demonstrate the potential this method holds.

The quantum computer has fuelled the imagination of scientists for decades: It is based on fundamentally different phenomena than a conventional computer....

Im Focus: Turmoil in sluggish electrons’ existence

An international team of physicists has monitored the scattering behaviour of electrons in a non-conducting material in real-time. Their insights could be beneficial for radiotherapy.

We can refer to electrons in non-conducting materials as ‘sluggish’. Typically, they remain fixed in a location, deep inside an atomic composite. It is hence...

Im Focus: Wafer-thin Magnetic Materials Developed for Future Quantum Technologies

Two-dimensional magnetic structures are regarded as a promising material for new types of data storage, since the magnetic properties of individual molecular building blocks can be investigated and modified. For the first time, researchers have now produced a wafer-thin ferrimagnet, in which molecules with different magnetic centers arrange themselves on a gold surface to form a checkerboard pattern. Scientists at the Swiss Nanoscience Institute at the University of Basel and the Paul Scherrer Institute published their findings in the journal Nature Communications.

Ferrimagnets are composed of two centers which are magnetized at different strengths and point in opposing directions. Two-dimensional, quasi-flat ferrimagnets...

Im Focus: World's thinnest hologram paves path to new 3-D world

Nano-hologram paves way for integration of 3-D holography into everyday electronics

An Australian-Chinese research team has created the world's thinnest hologram, paving the way towards the integration of 3D holography into everyday...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Marine Conservation: IASS Contributes to UN Ocean Conference in New York on 5-9 June

24.05.2017 | Event News

AWK Aachen Machine Tool Colloquium 2017: Internet of Production for Agile Enterprises

23.05.2017 | Event News

Dortmund MST Conference presents Individualized Healthcare Solutions with micro and nanotechnology

22.05.2017 | Event News

 
Latest News

How herpesviruses win the footrace against the immune system

26.05.2017 | Life Sciences

Water forms 'spine of hydration' around DNA, group finds

26.05.2017 | Life Sciences

First Juno science results supported by University of Leicester's Jupiter 'forecast'

26.05.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>