Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Heidelberg University researcher awarded Animal Welfare Prize of the German Research Foundation

19.03.2014

New methods for vascular research reduce number of experimental animals / Human umbilical cord cells mimic vessel walls

Professor Thomas Korff of the Department of Physiology and Pathophysiology at Heidelberg University, Germany, was awarded the German Research Foundation (DFG)’s Ursula M. Händel Animal Welfare Prize on March 20, 2014, at a ceremony in Berlin.


Professor Thomas Korff developed a model system for human blood vessels in the petri dish.

Photo: Heidelberg University Hospital

The physiologist investigates the formation and remodeling of blood vessels and has developed methods that minimize the distress experienced by animals and reduce the number of test animals. In certain areas, animal experiments can be replaced completely.

Professor Korff plans to use the prize money in the amount of 100,000 euros to refine his methods and standardize them so that they can be introduced and applied in other research laboratories without a great deal of effort.

Blood vessels in the petri dish

The award winner and his group study the processes and mechanisms in blood vessels that underlie normal development as well as pathological remodeling processes associated with, e.g. atherosclerosis or varicose veins. In order to use human cells for his experiments to the greatest extent possible, Korff has developed special culture methods.

To this end, he cultivates spherical cellular aggregates from cells that are isolated from the blood vessels of human umbilical cord after birth. These cell masses mimic two layers of the vessel wall. 

This model system for human blood vessels is not only well suited for basic research, but is now also being used in industrial applications. Scientists from Beiersdorf AG in Hamburg, Germany, are using the model system to test the protective effect of cosmetic substances on microscopic skin vessels. “Since we categorically excludeanimal testing, we use these kinds of realistic methods with human cells, which are especially significant for us,” said Dr. GittaNeufang, Head of Medical Management at Beiersdorf AG.

Test mice affected as little as possible

“However, cell cultures reach their limits for applications beyond cosmetics, for instance, if we want to find out how and why vessels undergo pathological changes,” Korff pointed out. In this case, it is not possible to avoid direct manipulations on animals, he clarified. “However, we have developed new surgical techniques that are much less stressful for the test animal than other proceduresused in vascular research.”

The new methods are easy to perform and mean less distress for the animals. “The animals behave normally and the success rate of the surgical procedures is higher. We need fewer animals for reliable results, which also reduces the costs,” he added.

For experiments on the living organism, the team often uses the ear of the mouse, in which the blood vessels are already clearly visible with the naked eye. The mouse ear is also easily accessible for many imaging techniquesand is suitable as a model for investigating many research questions. Without a single incision, for instance, a vein can be tied off in order to raise blood pressure in the afferent vessels.

In so doing, the formation of varicose veins can be simulated and their development observed over a period of several days. With this model, Korff investigates what signal pathways and molecules promote the pathological enlargement of the veins and whether certain substances can influence it. These kinds of studies are essential for identifying approaches for future therapeutic treatment.

In another project, the research group uses the mouse ear to investigate how tumors influence existing vessels or stimulate the formation of new vessels and, in so doing, can ensure their own blood supply. “Processes that are so complex can only be studied in live animals,” Korff explained.

Literature:
Stretch-induced activation of the transcription factor activator protein-1 controls monocyte chemoattractant protein-1 expression during arteriogenesis. Demicheva E, Hecker M, Korff T. Circ Res. 2008 Aug 29;103(5):477-84.

Feldner A, Otto H, Rewerk S, Hecker M, Korff T. Experimental hypertension triggers varicosis-like maladaptive venous remodeling through activator protein-1. FASEB J. 2011 Oct;25(10):3613-21.

Navid F, Kolbe L, Stäb F, Korff T, Neufang G. UV-radiation induces the release of angiopoietin-2 from dermal microvascular endothelial cells. ExpDermatol. 2012 Feb;21(2):147-53.

More information is available on the Web:
Working group Professor Thomas Korff: http://www.medizinische-fakultaet-hd.uni-heidelberg.de/Gruppe-Korff.110926.0.htm...
Ursula M. Händel Animal Welfare Prize: www.dfg.de/haendel-preis
DFG press release: www.dfg.de/service/presse/pressemitteilungen/2014/pressemitteilung_nr_04/index.html

Contact for journalists:
Dr. Gerd König
Institute of Physiology and Pathophysiology
Division of Cardiovascular Physiology
Heidelberg University
Tel. +49 6221 54-4067 or +49 1525 3502007
E-mail: gerd.koenig@physiologie.uni-heidelberg.de

Heidelberg University Hospital and Medical Faculty:
Internationally recognized patient care, research, and teaching

Heidelberg University Hospital is one of the largest and most prestigious medical centers in Germany. The Medical Faculty of Heidelberg University belongs to the internationally most renowned biomedical research institutions in Europe. Both institutions have the common goal of developing new therapies and implementing them rapidly for patients. With about 11,000 employees, training and qualification is an important issue. Every year, around 118,000 patients are treated on an inpatient basis and around 1.000.000 cases on an outpatient basis in more than 50 clinics and departments with 2,200 beds. Currently, about 3,500 future physicians are studying in Heidelberg; the reform Heidelberg Curriculum Medicinale (HeiCuMed) is one of the top medical training programs in Germany.

Requests by journalists:
Dr. Annette Tuffs
Director, Corporate Communication/Public Relations
University Hospital and
Medical Faculty of Heidelberg
Im Neuenheimer Feld 672
69120 Heidelberg
Germany
phone: +49 6221 / 56 45 36
fax: +49 6221 / 56 45 44
e-mail: annette.tuffs@med.uni-heidelberg.de

Selected english press releases online:
http://www.klinikum.uni-heidelberg.de/presse

Dr. Annette Tuffs | idw - Informationsdienst Wissenschaft

More articles from Awards Funding:

nachricht Helmholtz International Fellow Award for Sarah Amalia Teichmann
20.01.2017 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt

nachricht Scientist from Kiel University coordinates Million Euros Project in Inflammation Research
19.01.2017 | Christian-Albrechts-Universität zu Kiel

All articles from Awards Funding >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Quantum optical sensor for the first time tested in space – with a laser system from Berlin

For the first time ever, a cloud of ultra-cold atoms has been successfully created in space on board of a sounding rocket. The MAIUS mission demonstrates that quantum optical sensors can be operated even in harsh environments like space – a prerequi-site for finding answers to the most challenging questions of fundamental physics and an important innovation driver for everyday applications.

According to Albert Einstein's Equivalence Principle, all bodies are accelerated at the same rate by the Earth's gravity, regardless of their properties. This...

Im Focus: Traffic jam in empty space

New success for Konstanz physicists in studying the quantum vacuum

An important step towards a completely new experimental access to quantum physics has been made at University of Konstanz. The team of scientists headed by...

Im Focus: How gut bacteria can make us ill

HZI researchers decipher infection mechanisms of Yersinia and immune responses of the host

Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...

Im Focus: Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.

While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...

Im Focus: Studying fundamental particles in materials

Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales

Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Sustainable Water use in Agriculture in Eastern Europe and Central Asia

19.01.2017 | Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

 
Latest News

Tracking movement of immune cells identifies key first steps in inflammatory arthritis

23.01.2017 | Health and Medicine

Electrocatalysis can advance green transition

23.01.2017 | Physics and Astronomy

New technology for mass-production of complex molded composite components

23.01.2017 | Process Engineering

VideoLinks
B2B-VideoLinks
More VideoLinks >>>