To calculate microbes in milk or in the operating-room, it is necessary to make them glow like well-known glow-beetles (Lampyridae gen.) do. Now that the secret of glow-beetles’ luminescence has been discovered, and researchers of the Lomonosov Moscow State University not only synthesize the enzyme required for luminescence, but they also produce snap analysis sets based on it, the sets allowing to literally calculate microbes practically in any analysis object within several minutes. The most complicated cases would require the six-hour analysis.
“Our method is based on measuring intensity of luminescence, which occurs upon interaction of a special enzyme, luciferase, with molecules of the so-called ATF - adenosinetriphosphoric acid, says Professor Ugarova, (Doctor of Science (Chemistry)), research manager. This substance called universal accumulator and energy source of living organisms exists in any bioplast. Accordingly, it does exist in microbes as well.”
If membranes of microbe’s sells are destroyed, ATF will, bluntly speaking, simply flow out of them and its quantity may be determined with the help of luciferase. The relation is direct: the more bioplast the sample contains, the more ATF is contained in the solution and the more it will glow upon addition of the enzyme. Certainly, this is a highly simplified outline of the process, and the way from the first investigations up to a simple and reliable methodology took years. However, it has been almost passed through, at any rate the first pilot batches of snap analysis sets have already been released.
Sergey Komarov | alfa
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