Microbiologist Hong Li and veterinary medical officer Naomi Taus at the ARS Animal Diseases Research Unit in Pullman, Wash., collaborated on the research with Lindsay Oaks at Washington State University and Donal O'Toole at the University of Wyoming.
MCF, a viral infection that is a leading cause of disease in American bison, is usually transmitted from sheep to bison and cattle. Vaccine development has been stymied because the virus won't grow in cell culture.
The ARS scientists and their university colleagues have shown that the virus undergoes several changes inside the animal's body, targeting specific cell types at different stages of its own life cycle. This process is called "cell tropism switching."
The viral replication in sheep can be divided into three stages: entry, maintenance, and shedding. The virus enters the sheep through its nasal passages and reaches the lungs, where it replicates. Replication in the sheep lung is required for the virus to change its cell tropism for the next stage: the infection of lymphocytes, a type of immune cell.
During this maintenance stage, the virus stays in the lymphocytes that circulate through the whole body, with little replication. This type of infection is referred to as a "latent infection." During the shedding stage, the virus reactivates from the infected lymphocytes and targets specific cells in the nasal area to complete its replication. The virus is then shed through the sheep's nasal secretions.
Now that they understand these viral changes, scientists can begin to find the right cell types to grow the virus in cell culture, according to Li.
Read more about this research in the April 2010 issue of Agricultural Research magazine.
ARS is the principal intramural scientific research agency of the U.S. Department of Agriculture (USDA). The research supports the USDA priority of promoting international food security.
Sharon Durham | EurekAlert!
How much drought can a forest take?
20.01.2017 | University of California - Davis
Plasma-zapping process could yield trans fat-free soybean oil product
02.12.2016 | Purdue University
An important step towards a completely new experimental access to quantum physics has been made at University of Konstanz. The team of scientists headed by...
Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...
Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.
While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...
Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales
Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...
Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.
As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...
19.01.2017 | Event News
10.01.2017 | Event News
09.01.2017 | Event News
20.01.2017 | Awards Funding
20.01.2017 | Materials Sciences
20.01.2017 | Life Sciences