Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Research About Plant Viruses Could Lead to New Ways to Improve Crop Yields

06.10.2008
An interdisciplinary group of scientists has obtained the first detailed information about the structure of the most destructive group of plant viruses known: flexible filamentous viruses.

The cost of worldwide crop losses due to plant diseases is estimated at $60 billion annually. Although there are no good estimates of the cost of plant viruses alone, the viruses are generally considered to be the second greatest contributor to those losses (after fungi). The 300-plus species of flexible filamentous viruses are responsible for more than half of all virus damage.

The findings are published in the October 1 issue of the Journal of Virology and could lead to new ways to protect crop plants from these viruses. The structural information that the researchers have obtained may also benefit researchers interested in using viruses as agents of biotechnology to coax plants to produce other useful products, such as pharmaceuticals.

“These are very important plant viruses, and we knew almost nothing about their detailed structure before these studies,” says Gerald Stubbs, the professor of biological sciences at Vanderbilt University who directed the study. “Their flexibility made them very difficult to analyze.”

The project took more than five years to complete and required the combined skills of scientists from Vanderbilt, the Department of Energy’s Brookhaven National Laboratory and Argonne National Laboratory, Boston University, Illinois Institute of Technology and the University of Kentucky using a combination of complementary imaging techniques. The result of this effort was determination of the low-resolution structures of two species of flexible filamentous viruses: soybean mosaic virus and potato virus X.

Amy Kendall, Michele McDonald, Wen Bian, Timothy Bowles, Sarah Baumgarten, Jian Shi and Phoebe Stewart from Vanderbilt; Esther Bullitt from Boston University School of Medicine; David Gore and Thomas Irving from Illinois Institute of Technology; Wendy Havens and Said Ghabrial from the University of Kentucky and Joseph Wall from Brookhaven National Laboratory all contributed to the study.

The researchers found that the external protein coats of the two viruses are extremely similar even though they come from two different viral families, Potyviridae and Flexiviridae.

“Although the two viruses are unrelated, they have the same outer protein coat,” says Stubbs. “Sometime in the past, a member of one family must have obtained the coat protein gene from a member of the other family, and it worked so well that pretty soon it was everywhere.”

The exchange of genes between different viruses is a well-known phenomenon and generally takes place when two viruses invade the same host, Stubbs says. According to the scientist, this makes it quite likely that a third family of flexible filamentous viruses, the Closteroviridae, has the same protein coat as well.

“Actually, the coat protein is not particularly good at protecting the genome,” says Stubbs. “It must confer some evolutionary advantage, but we don’t know what that might be.”

The viruses are much too small to see with an optical microscope. So the scientists had to use a combination of imaging techniques — cryo-electron microscopy at Vanderbilt, X-ray diffraction at Argonne and scanning transmission electron microscopy (STEM) at Brookhaven – in order to identify the two structures.

“These techniques are very complementary,” says Stubbs. “People have been trying to get this structural work started for decades, more than 40 years. It’s been very difficult and there have been a number of obstacles, including the fact that it’s very hard to make good samples of these viruses.”

Even after Stubbs and his colleagues figured out how to make good samples and analyzed them using X-ray diffraction and traditional electron microscopy, there were a number of ambiguities in the results. Brookhaven’s STEM technique provided the definitive answers. Although it could not determine the structure of the viral protein coat directly, STEM was able to put boundaries on the number of molecules in each “turn” of the spiral-shaped structure, and this allowed the scientists to select the correct structure from the alternatives they had come up with using the other techniques.

Some potential applications of these results include engineering molecules based on the viral structure that interfere with the viruses’ ability to infect plants. Another possibility would be to introduce modified versions of viruses or to coat protein genes into a plant to protect it from viral and insect attacks. Yet another possible application would be to use modified viruses to introduce genes that instruct plants to make other useful products — for example, antibiotics or other drugs.

The research project was funded by grants from the National Science Foundation, Department of Agriculture, Department of Energy and National Institutes of Health.

David F. Salisbury | Newswise Science News
Further information:
http://www.vanderbilt.edu
http://www.vanderbilt.edu/exploration/stories/filamentous.html

More articles from Agricultural and Forestry Science:

nachricht New 3-D model predicts best planting practices for farmers
26.06.2017 | Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign

nachricht Fighting a destructive crop disease with mathematics
21.06.2017 | University of Cambridge

All articles from Agricultural and Forestry Science >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Can we see monkeys from space? Emerging technologies to map biodiversity

An international team of scientists has proposed a new multi-disciplinary approach in which an array of new technologies will allow us to map biodiversity and the risks that wildlife is facing at the scale of whole landscapes. The findings are published in Nature Ecology and Evolution. This international research is led by the Kunming Institute of Zoology from China, University of East Anglia, University of Leicester and the Leibniz Institute for Zoo and Wildlife Research.

Using a combination of satellite and ground data, the team proposes that it is now possible to map biodiversity with an accuracy that has not been previously...

Im Focus: Climate satellite: Tracking methane with robust laser technology

Heatwaves in the Arctic, longer periods of vegetation in Europe, severe floods in West Africa – starting in 2021, scientists want to explore the emissions of the greenhouse gas methane with the German-French satellite MERLIN. This is made possible by a new robust laser system of the Fraunhofer Institute for Laser Technology ILT in Aachen, which achieves unprecedented measurement accuracy.

Methane is primarily the result of the decomposition of organic matter. The gas has a 25 times greater warming potential than carbon dioxide, but is not as...

Im Focus: How protons move through a fuel cell

Hydrogen is regarded as the energy source of the future: It is produced with solar power and can be used to generate heat and electricity in fuel cells. Empa researchers have now succeeded in decoding the movement of hydrogen ions in crystals – a key step towards more efficient energy conversion in the hydrogen industry of tomorrow.

As charge carriers, electrons and ions play the leading role in electrochemical energy storage devices and converters such as batteries and fuel cells. Proton...

Im Focus: A unique data centre for cosmological simulations

Scientists from the Excellence Cluster Universe at the Ludwig-Maximilians-Universität Munich have establised "Cosmowebportal", a unique data centre for cosmological simulations located at the Leibniz Supercomputing Centre (LRZ) of the Bavarian Academy of Sciences. The complete results of a series of large hydrodynamical cosmological simulations are available, with data volumes typically exceeding several hundred terabytes. Scientists worldwide can interactively explore these complex simulations via a web interface and directly access the results.

With current telescopes, scientists can observe our Universe’s galaxies and galaxy clusters and their distribution along an invisible cosmic web. From the...

Im Focus: Scientists develop molecular thermometer for contactless measurement using infrared light

Temperature measurements possible even on the smallest scale / Molecular ruby for use in material sciences, biology, and medicine

Chemists at Johannes Gutenberg University Mainz (JGU) in cooperation with researchers of the German Federal Institute for Materials Research and Testing (BAM)...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Plants are networkers

19.06.2017 | Event News

Digital Survival Training for Executives

13.06.2017 | Event News

Global Learning Council Summit 2017

13.06.2017 | Event News

 
Latest News

Predicting eruptions using satellites and math

28.06.2017 | Earth Sciences

Extremely fine measurements of motion in orbiting supermassive black holes

28.06.2017 | Physics and Astronomy

Touch Displays WAY-AX and WAY-DX by WayCon

27.06.2017 | Power and Electrical Engineering

VideoLinks
B2B-VideoLinks
More VideoLinks >>>