Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

MTT’s barley gene transfer technique ready

03.12.2007
MTT Agrifood Research Finland has introduced an Agrobacterium-mediated gene transfer technique for barley. Genetically modified barley is at the same time the main opening in plant gene transfer research at MTT.

Development of gene transfer methods forms part of the Disease Resistant Barley project, which provides genomic tools for managing and monitoring barley net blotch. The project, which started in 2005 and runs until 2011, is implemented in cooperation between MTT, the University of Helsinki, and Boreal Plant Breeding Ltd.

In addition to gene transfer, our research focuses include gene mapping, isolation of genes on the basis of map location, new gene replication methods, and bioinformatics.

Net blotch is the number one disease afflicting barley in Finland. Although the area of the barley genome concerned with resistance to net blotch has been identified, there is no accurate information on the genes located there. Gene transfer enables us to identify and verify which genes leads to net blotch resistance in barley, and how these genes function.

– Once the gene is identified, we can use methods for plant breeding in which this characteristic can be identified from wild barley strains, for example, and new barley varieties produced through an intensified back cross programme, says Outi Manninen, the research scientist responsible for the Disease Resistant Barley project.

– Genomics makes use of gene transfer as a tool, and the production of a genetically modified (GM) variety of barley is not the actual objective. New information gleaned from the research can nevertheless be exploited in the development of GM varieties.

Marker genes transferred

The MTT plant genomics team employed Agrobacterium as the means of gene transfer. Agrobacterium is a general soil bacterium with an excellent natural capacity for gene transfer. The gene transferred to barley is a marker gene producing fluorescent protein, the colour helping to track gene function.

The project has utilized retrotransposon promoters areas isolated from the barley genome, which have been extensively researched by MTT research scientist Professor Alan Schulman. Promoters have a significant role in gene function, as they regulate at what intensity, what stage and in which parts of the plant the genes will function.

Promoter areas used in transfers are mostly derived from maize or the cauliflower mosaic virus. It is hoped the usual phenomenon of promoters turning off if many gene copies are transferred simultaneously will be avoided with the aid of new Finnish applications.

Sufficiently efficient

Outi Manninen says that all plants produced from embryo cell tissue in MTT research have proved to be genetically modified. – The method has now acquired an ample degree of efficiency and is very effective.

Juha Heikkilae | alfa
Further information:
http://www.mtt.fi/english/

More articles from Agricultural and Forestry Science:

nachricht New insight into why Pierce's disease is so deadly to grapevines
11.06.2018 | University of California - Davis

nachricht Where are Europe’s last primary forests?
29.05.2018 | Humboldt-Universität zu Berlin

All articles from Agricultural and Forestry Science >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Temperature-controlled fiber-optic light source with liquid core

In a recent publication in the renowned journal Optica, scientists of Leibniz-Institute of Photonic Technology (Leibniz IPHT) in Jena showed that they can accurately control the optical properties of liquid-core fiber lasers and therefore their spectral band width by temperature and pressure tuning.

Already last year, the researchers provided experimental proof of a new dynamic of hybrid solitons– temporally and spectrally stationary light waves resulting...

Im Focus: Overdosing on Calcium

Nano crystals impact stem cell fate during bone formation

Scientists from the University of Freiburg and the University of Basel identified a master regulator for bone regeneration. Prasad Shastri, Professor of...

Im Focus: AchemAsia 2019 will take place in Shanghai

Moving into its fourth decade, AchemAsia is setting out for new horizons: The International Expo and Innovation Forum for Sustainable Chemical Production will take place from 21-23 May 2019 in Shanghai, China. With an updated event profile, the eleventh edition focusses on topics that are especially relevant for the Chinese process industry, putting a strong emphasis on sustainability and innovation.

Founded in 1989 as a spin-off of ACHEMA to cater to the needs of China’s then developing industry, AchemAsia has since grown into a platform where the latest...

Im Focus: First real-time test of Li-Fi utilization for the industrial Internet of Things

The BMBF-funded OWICELLS project was successfully completed with a final presentation at the BMW plant in Munich. The presentation demonstrated a Li-Fi communication with a mobile robot, while the robot carried out usual production processes (welding, moving and testing parts) in a 5x5m² production cell. The robust, optical wireless transmission is based on spatial diversity; in other words, data is sent and received simultaneously by several LEDs and several photodiodes. The system can transmit data at more than 100 Mbit/s and five milliseconds latency.

Modern production technologies in the automobile industry must become more flexible in order to fulfil individual customer requirements.

Im Focus: Sharp images with flexible fibers

An international team of scientists has discovered a new way to transfer image information through multimodal fibers with almost no distortion - even if the fiber is bent. The results of the study, to which scientist from the Leibniz-Institute of Photonic Technology Jena (Leibniz IPHT) contributed, were published on 6thJune in the highly-cited journal Physical Review Letters.

Endoscopes allow doctors to see into a patient’s body like through a keyhole. Typically, the images are transmitted via a bundle of several hundreds of optical...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Munich conference on asteroid detection, tracking and defense

13.06.2018 | Event News

2nd International Baltic Earth Conference in Denmark: “The Baltic Sea region in Transition”

08.06.2018 | Event News

ISEKI_Food 2018: Conference with Holistic View of Food Production

05.06.2018 | Event News

 
Latest News

Better model of water under extreme conditions could aid understanding of Earth's mantle

21.06.2018 | Earth Sciences

What are the effects of coral reef marine protected areas?

21.06.2018 | Life Sciences

The Janus head of the South Asian monsoon

21.06.2018 | Earth Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>