Reseachers at The Ohio State University have demonstrated that Target Region Amplification Polymorphism, or TRAP, is an effective method for preserving the important genetic diversity of ornamental flower collections.
Pelargonium, commonly know as geranium, are some of the most popular flowers the world. So popular, in fact, that the Royal Horticultural Society listed more than 3,000 varieties of geranium in their 2004 distribution catalogue. Sold in hanging baskets, flats and decorative pots, geranium plants accounted for more than $206 million in wholesale revenue in the U.S. during 2004. Essential oils from some scented geraniums are finding new uses in perfumes and food flavorings.
There are over 280 documented species of Pelargonium. The interest in breeding has resulted in many novel and improved cultivars. According to Rose Palumbo, Department of Plant Pathology at The Ohio State University (OSU), ornamental plants like the geranium are often bred for their ornamental qualities rather than their ability to survive in diverse environments.
Palumbo and a team of researchers recently completed a study of Pelargonium grown at The Ornamental Plant Germplasm Center (OPGC) at Ohio State. OPGC collects heirloom cultivars, breeding lines and wild species.
Intending to find a way to preserve the genetic diversity of Pelagonium, the team tested a method known as Target Region Amplification Polymorphism, or TRAP. Palumbo explained, "The TRAP method uses molecuar markers targeted to a specific gene. Target sequences that have been generally successful in most plants tested the applicability of this method to OSU's geranium collection. Using TRAP allowed us to divide the population into groups of similiar species and groups known to share parents."
Palumbo continued, "TRAP has the advantage of producing a large number of markers through use of sequence information that is already available. Our first goal was to determine the feasibility of TRAP for the analysis of this large collection, so that in the future the most diverse genotypes may be retained. To achieve this goal, we first modified existing DNA extraction techniques to account for the high levels of phenolic compounds present in some Pelargonium species. Second, we evaluated the TRAP procedure using the DNA isolated from 46 accessions. Based on these results, the molecular analysis of the collection was completed, and the collection has decreased in size by approximately 25%. Continuing analyses should shrink the collection from approximately 800 plants to close to 200 plants by the end of this year."
Using the TRAP method will allow the OPGC to streamline their collection of geraniums into a much more manageable size. According to Palumbo, the smaller collection will be more efficient to maintain, while still providing the diverse genetic resources needed by breeders and researchers.
Michael W. Neff | EurekAlert!
Climate change, population growth may lead to open ocean aquaculture
05.10.2017 | Oregon State University
New machine evaluates soybean at harvest for quality
04.10.2017 | University of Illinois College of Agricultural, Consumer and Environmental Sciences
Salmonellae are dangerous pathogens that enter the body via contaminated food and can cause severe infections. But these bacteria are also known to target...
University of Maryland researchers contribute to historic detection of gravitational waves and light created by event
On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...
Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.
Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....
Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).
When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...
Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.
How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...
23.10.2017 | Event News
17.10.2017 | Event News
10.10.2017 | Event News
23.10.2017 | Life Sciences
23.10.2017 | Physics and Astronomy
23.10.2017 | Health and Medicine