Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Coffee in vitro plantlets enter the industrial age

29.01.2008
The Ecom Industrial group's laboratory in Sebacco, Nicaragua, produced a million coffee in vitro plantlets in 2007. This figure had never previously been achieved for Arabica coffee, and is the fruit of the industrial-scale development of techniques built by CIRAD over more than 20 years.

The techniques centre on two major innovations that have served to increase the efficiency and productivity of in vitro propagation by somatic embryogenesis*. First of all, CIRAD's researchers developed a technique for mass producing pregerminated somatic embryos in Rita®-type bioreactors: the embryos are immersed in a nutrient solution for just a few minutes a day, up to a very advanced stage of germination. The team then developed a technique for sowing the in vitro plantlets produced in bioreactors directly on a horticultural substrate, for the first time in the field of in vitro culture.

In Nicaragua, the Ecom/CIRAD team has now succeeded in transferring somatic embryogenesis to an industrial scale. The aim was to achieve production costs for producers similar to those of plants grown from seed. As regards production in a liquid medium, there are now 3500 bioreactors producing 250 000 pregerminated embryos a month, which can be acclimatized directly to nursery conditions. Using liquid nutrient media has made it possible to automate the procedure and reduce production costs considerably. As regards planting the somatic embryos, weaning tunnels and acclimatization procedures have been adapted to this highly immature, very small (8-10 mm) type of material. More than 70% of the embryos germinate and grow into plants a few weeks after sowing. Sowing the somatic embryos directly also reduces work times and the space required in the laboratory, since germination and plantlet growth take place in the nursery and not in vitro. Production costs are thus considerably lower than with conventional procedures.

The CIRAD team is now continuing its research on somatic embryogenesis, but this time under production conditions. While supporting the change of scale and the transfer of this procedure to other countries, it is concentrating on how to synchronize embryo development in bioreactors and on guaranteeing that the regenerated in vitro plantlets are genetically true-to-type. As for the Sebacco laboratory and its staff of 40, the target for 2008 is to produce 2.4 million plants.

* Somatic embryogenesis is an in vitro culture technique that generates large quantities of somatic embryos, ie embryos not produced from seed, that germinate like seed embryos and grow into genetically identical trees (clonal reproduction). In coffee, this enables the rapid regeneration of several tens of thousands of trees identical to the mother tree, from a single leaf fragment placed in vitro.

Helen Burford | alfa
Further information:
http://www.cirad.fr/en/actualite/communique.php?id=855

More articles from Agricultural and Forestry Science:

nachricht Six-legged livestock -- sustainable food production
11.05.2017 | Faculty of Science - University of Copenhagen

nachricht Elephant Herpes: Super-Shedders Endanger Young Animals
04.05.2017 | Universität Zürich

All articles from Agricultural and Forestry Science >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: A quantum walk of photons

Physicists from the University of Würzburg are capable of generating identical looking single light particles at the push of a button. Two new studies now demonstrate the potential this method holds.

The quantum computer has fuelled the imagination of scientists for decades: It is based on fundamentally different phenomena than a conventional computer....

Im Focus: Turmoil in sluggish electrons’ existence

An international team of physicists has monitored the scattering behaviour of electrons in a non-conducting material in real-time. Their insights could be beneficial for radiotherapy.

We can refer to electrons in non-conducting materials as ‘sluggish’. Typically, they remain fixed in a location, deep inside an atomic composite. It is hence...

Im Focus: Wafer-thin Magnetic Materials Developed for Future Quantum Technologies

Two-dimensional magnetic structures are regarded as a promising material for new types of data storage, since the magnetic properties of individual molecular building blocks can be investigated and modified. For the first time, researchers have now produced a wafer-thin ferrimagnet, in which molecules with different magnetic centers arrange themselves on a gold surface to form a checkerboard pattern. Scientists at the Swiss Nanoscience Institute at the University of Basel and the Paul Scherrer Institute published their findings in the journal Nature Communications.

Ferrimagnets are composed of two centers which are magnetized at different strengths and point in opposing directions. Two-dimensional, quasi-flat ferrimagnets...

Im Focus: World's thinnest hologram paves path to new 3-D world

Nano-hologram paves way for integration of 3-D holography into everyday electronics

An Australian-Chinese research team has created the world's thinnest hologram, paving the way towards the integration of 3D holography into everyday...

Im Focus: Using graphene to create quantum bits

In the race to produce a quantum computer, a number of projects are seeking a way to create quantum bits -- or qubits -- that are stable, meaning they are not much affected by changes in their environment. This normally needs highly nonlinear non-dissipative elements capable of functioning at very low temperatures.

In pursuit of this goal, researchers at EPFL's Laboratory of Photonics and Quantum Measurements LPQM (STI/SB), have investigated a nonlinear graphene-based...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Marine Conservation: IASS Contributes to UN Ocean Conference in New York on 5-9 June

24.05.2017 | Event News

AWK Aachen Machine Tool Colloquium 2017: Internet of Production for Agile Enterprises

23.05.2017 | Event News

Dortmund MST Conference presents Individualized Healthcare Solutions with micro and nanotechnology

22.05.2017 | Event News

 
Latest News

Physicists discover mechanism behind granular capillary effect

24.05.2017 | Physics and Astronomy

Measured for the first time: Direction of light waves changed by quantum effect

24.05.2017 | Physics and Astronomy

Marine Conservation: IASS Contributes to UN Ocean Conference in New York on 5-9 June

24.05.2017 | Event News

VideoLinks
B2B-VideoLinks
More VideoLinks >>>