Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Coffee in vitro plantlets enter the industrial age

29.01.2008
The Ecom Industrial group's laboratory in Sebacco, Nicaragua, produced a million coffee in vitro plantlets in 2007. This figure had never previously been achieved for Arabica coffee, and is the fruit of the industrial-scale development of techniques built by CIRAD over more than 20 years.

The techniques centre on two major innovations that have served to increase the efficiency and productivity of in vitro propagation by somatic embryogenesis*. First of all, CIRAD's researchers developed a technique for mass producing pregerminated somatic embryos in Rita®-type bioreactors: the embryos are immersed in a nutrient solution for just a few minutes a day, up to a very advanced stage of germination. The team then developed a technique for sowing the in vitro plantlets produced in bioreactors directly on a horticultural substrate, for the first time in the field of in vitro culture.

In Nicaragua, the Ecom/CIRAD team has now succeeded in transferring somatic embryogenesis to an industrial scale. The aim was to achieve production costs for producers similar to those of plants grown from seed. As regards production in a liquid medium, there are now 3500 bioreactors producing 250 000 pregerminated embryos a month, which can be acclimatized directly to nursery conditions. Using liquid nutrient media has made it possible to automate the procedure and reduce production costs considerably. As regards planting the somatic embryos, weaning tunnels and acclimatization procedures have been adapted to this highly immature, very small (8-10 mm) type of material. More than 70% of the embryos germinate and grow into plants a few weeks after sowing. Sowing the somatic embryos directly also reduces work times and the space required in the laboratory, since germination and plantlet growth take place in the nursery and not in vitro. Production costs are thus considerably lower than with conventional procedures.

The CIRAD team is now continuing its research on somatic embryogenesis, but this time under production conditions. While supporting the change of scale and the transfer of this procedure to other countries, it is concentrating on how to synchronize embryo development in bioreactors and on guaranteeing that the regenerated in vitro plantlets are genetically true-to-type. As for the Sebacco laboratory and its staff of 40, the target for 2008 is to produce 2.4 million plants.

* Somatic embryogenesis is an in vitro culture technique that generates large quantities of somatic embryos, ie embryos not produced from seed, that germinate like seed embryos and grow into genetically identical trees (clonal reproduction). In coffee, this enables the rapid regeneration of several tens of thousands of trees identical to the mother tree, from a single leaf fragment placed in vitro.

Helen Burford | alfa
Further information:
http://www.cirad.fr/en/actualite/communique.php?id=855

More articles from Agricultural and Forestry Science:

nachricht How much drought can a forest take?
20.01.2017 | University of California - Davis

nachricht Plasma-zapping process could yield trans fat-free soybean oil product
02.12.2016 | Purdue University

All articles from Agricultural and Forestry Science >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Traffic jam in empty space

New success for Konstanz physicists in studying the quantum vacuum

An important step towards a completely new experimental access to quantum physics has been made at University of Konstanz. The team of scientists headed by...

Im Focus: How gut bacteria can make us ill

HZI researchers decipher infection mechanisms of Yersinia and immune responses of the host

Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...

Im Focus: Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.

While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...

Im Focus: Studying fundamental particles in materials

Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales

Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...

Im Focus: Designing Architecture with Solar Building Envelopes

Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.

As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Sustainable Water use in Agriculture in Eastern Europe and Central Asia

19.01.2017 | Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

 
Latest News

Helmholtz International Fellow Award for Sarah Amalia Teichmann

20.01.2017 | Awards Funding

An innovative high-performance material: biofibers made from green lacewing silk

20.01.2017 | Materials Sciences

Ion treatments for cardiac arrhythmia — Non-invasive alternative to catheter-based surgery

20.01.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>