Rice containing an overactive gene that makes it resistant to a common herbicide can pass that genetic trait to weedy rice, prompting powerful growth even without a weed-killer to trigger the modification benefit, new research shows.
Previously, scientists have found that when a genetically modified trait passes from a crop plant to a closely related weed, the weed gains the crop’s engineered benefit – resistance to pests, for example – only in the presence of the offending insects.
This new study is a surprising example of gene flow from crops to weeds that makes weeds more vigorous even without an environmental trigger, researchers say.
The suspected reason: This modification method enhances a plant’s own growth control mechanism, essentially making it grow faster – an attractive trait in crops but a recipe for potential problems with weedy relatives that could out-compete the crop.
“Our next question is whether this method of enhancing plant growth could be developed for any crop. We want to know whether growers could get higher yields in the crop and then, if it happened to cross with a related weed, whether it might make the weed more prolific as well,” said Allison Snow, professor of evolution, ecology and organismal biology at The Ohio State University and a lead author of the paper.
“It’s unusual for any transgene to have such a positive effect on a wild relative and even more so for herbicide resistance,” she said. “But we think we know why: It’s probably because the pathway regulated by this gene is so important to the plant.”
The work is the result of Snow’s longtime collaboration with senior author Bao-Rong Lu, a professor at Fudan University in Shanghai. Their publication appears online in the journal New Phytologist.
The weed-killer glyphosate, sold under the brand name Roundup, kills plants by inhibiting a growth-related pathway activated by the epsps gene. Biotech companies have inserted mutated forms of a similar gene from microbes into crop plants, producing “Roundup Ready” corn and soybeans that remain undamaged by widespread herbicide application.
But in this study, the researchers used a different method, boosting activation of the native epsps gene in rice plants – a process called overexpressing – to give the plants enough strength to survive an application of herbicide. Because companies that genetically modify commercial crops don’t fully disclose their methods, Snow and her colleagues aren’t sure how prevalent this method might be, now or in the future.
“This is a relatively new way to get a trait into a crop: taking the plant’s own gene and ramping it up,” Snow said. “We don’t know yet if our findings are going to be generalizable, but if they are, it’s definitely going to be important.”
To overexpress the native gene in rice, the scientists attached a promoter to it, giving the plant an extra copy of its own gene and ensuring that the gene is activated at all times.
The researchers conducted tests in rice and four strains of a relative of the same species, weedy rice, a noxious plant that infests rice fields around the world. By crossing genetically altered herbicide-resistant rice with weedy rice to mimic what happens naturally in the field, the researchers created crop-weed hybrids that grew larger and produced more offspring than unaltered counterparts – even without any herbicide present.
In regulated field experiments, the hybrids containing the overexpressed gene produced 48 percent to 125 percent more seeds per plant than did hybrid plants with no modified genes. They also had higher concentrations of a key amino acid, greater photosynthetic rates and better fledgling seed growth than controls – all presumed signs of better fitness in evolutionary terms.
“Fitness is a hard thing to measure, but you can conclude that if a gene gives you a lot more seeds per plant compared to controls, it’s likely to increase the plants’ fitness because those genes would be represented at a higher percentage in future generations,” Snow said.
When Snow and Lu set out to study this new genetic engineering method, they didn’t know what to expect.
“Our colleagues developed this novel transgenic trait in rice and we didn’t know if it would have a fitness benefit, or a cost, or be neutral,” Snow said. “With most types of herbicide resistant genes, there’s no benefit to a wild plant unless the herbicide is sprayed. A lot of transgenes in crop plants are either selectively neutral in wild plants or, if they have a benefit, it depends on environmental factors like insects, diseases or herbicides being present.”
Snow has a history in this area of research. She has found that genes from crop plants can persist in related weeds over many generations. In 2002, she led a study that was the first to show that a gene artificially inserted into crop plants to fend off pests could migrate to weeds in a natural environment and make the weeds stronger. She also has served on national panels that monitor and make recommendations about the release of genetically engineered species into the environment.
She is interested in identifying new possible outcomes of the growth of crop-weed hybrids that contain genetic modifications, but she doesn’t take sides about possible risks and benefits of genetically modified crops.
“It’s not always the end of the world if a weed starts to become a lot more common after acquiring a new trait – there may be effective ways to manage that weed,” Snow said. “You just can’t make sweeping generalizations about genetic engineering, and knowledge from ecological studies like ours can help inform risk assessment and biosafety oversight.”
This work was supported by the Natural Science Foundation of China, the “973” program, the National Program of Development of Transgenic New Species of China and Ohio State University.
Additional co-authors are Wei Wang, Hui Xia, Xiao Yang, Ting Xu, Hong Jiang Si and Xing Xing Cai of Fudan University, and Feng Wang and Jun Su of Fujian Academy of Agricultural Sciences in Fuzhou, China.Contact: Allison Snow, (614) 292-3445; Snow.firstname.lastname@example.org
Allison Snow | EurekAlert!
New data unearths pesticide peril in beehives
21.04.2017 | Cornell University
New rice fights off drought
04.04.2017 | RIKEN
The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.
Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...
The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...
Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.
Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...
Two researchers at Heidelberg University have developed a model system that enables a better understanding of the processes in a quantum-physical experiment...
Glaciers might seem rather inhospitable environments. However, they are home to a diverse and vibrant microbial community. It’s becoming increasingly clear that they play a bigger role in the carbon cycle than previously thought.
A new study, now published in the journal Nature Geoscience, shows how microbial communities in melting glaciers contribute to the Earth’s carbon cycle, a...
20.04.2017 | Event News
18.04.2017 | Event News
03.04.2017 | Event News
21.04.2017 | Physics and Astronomy
21.04.2017 | Health and Medicine
21.04.2017 | Physics and Astronomy