By using infrared temperature measurement, seed viability results are achieved in less than two hours. Until now time-consuming germination tests, taking up to three days, were used to assess seed quality. Conventional tests are also destructive, which is not ideal when assessing the seeds of rare and endangered plants.
Dr. Ilse Kranner, from Kew's Millennium Seed Bank, and Prof. Gerald Kastberger (University of Graz, Austria) found that infrared cameras can be used to detect subtle changes in temperature when seeds take up water. These changes vary with viability.
The thermal profiles of hundreds of garden pea seeds (Pisum sativum) were recorded. For each individual seed 22,000 images were analysed to construct a library of "thermal fingerprints" that allowed the scientists to distinguish between viable and dead seeds in less than two hours.
When a dry seed takes up water, the sugar within the seed dissolves, and this process cools the seed down. The temperature of a single pea seed falls rapidly by 2 to 3°C. Viable seeds maintain cool temperatures because they break down storage reserves into sugar.
In aged seeds, certain biophysical properties are affected that determine the speed of water uptake. Aged seeds also fail to break down their reserves, or can only break them down after a phase of repair, delaying the thermal profile. To date such processes were studied with destructive methods that involved grinding up seeds, whereas the new infrared-based method is a breakthrough in the non-invasive diagnosis of seed quality. It means viable seeds can be separated, re-dried and stored again.
Non-invasive techniques are widely used in medicine, for example CT scans of the body, and some airports also use infrared cameras to detect fever in passengers.
Importantly, the opportunity to select live and dead seeds prior to germination is a useful tool to improve studies into the fundamental principles of ageing and cell death, which are similar in plants, animals and humans. This research can potentially be applied in areas such as cancer research.
Wheat (Triticum aestivum) and rape seeds (Brassica napus) were also studied by the British-Austrian team to provide a proof of concept for agricultural seeds.
The seeds of 10% of the world's wild plant flora are safeguarded at Kew's Millennium Seed Bank at Wakehurst Place in West Sussex, with the aim to conserve a maximum of plant diversity for future generations.
Timelapse videos, showing the temperature changes in live and dead seeds, are available to view online www.pnas.org/cgi/doi/10.1073/pnas.0914197107
For more information and to set up interviews please contact Ilse Kranner at Kew's Millennium Seed Bank on +44(0)1444 894157 or email email@example.com
Notes to editorsNoninvasive diagnosis of seed viability using infrared thermography
Wakehurst Place is also home to Kew's Millennium Seed Bank, the largest wild plant seed bank in the world. RBG Kew and its partners have collected and conserved seed from 10 per cent of the world's wild flowering plant species (c. 30,000 species). The aim is to conserve 25% by 2020 and funds are being actively sought in order to continue to develop this vital work. For further information please visit www.kew.org/msbp
Ilse Kranner | EurekAlert!
Energy crop production on conservation lands may not boost greenhouse gases
13.03.2017 | Penn State
How nature creates forest diversity
07.03.2017 | International Institute for Applied Systems Analysis (IIASA)
The Institute of Semiconductor Technology and the Institute of Physical and Theoretical Chemistry, both members of the Laboratory for Emerging Nanometrology (LENA), at Technische Universität Braunschweig are partners in a new European research project entitled ChipScope, which aims to develop a completely new and extremely small optical microscope capable of observing the interior of living cells in real time. A consortium of 7 partners from 5 countries will tackle this issue with very ambitious objectives during a four-year research program.
To demonstrate the usefulness of this new scientific tool, at the end of the project the developed chip-sized microscope will be used to observe in real-time...
Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.
The results will be published on March 22 in the journal „Astronomy & Astrophysics“.
Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...
Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.
Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...
In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...
20.03.2017 | Event News
14.03.2017 | Event News
07.03.2017 | Event News
28.03.2017 | Physics and Astronomy
28.03.2017 | Health and Medicine
28.03.2017 | Life Sciences