Jatropha has seeds with high oil content. But the oil's potential as a biofuel is limited because, for large-scale production, this shrub-like plant needs the same amount of care and resources as crop plants.
"It is thought that Jatropha's future lies in further improvement of Jatropha for large-scale production on marginal, non-food croplands through breeding and/or biotechnology," said John E. Carlson, professor of molecular genetics at Penn State. "The more that is known about the genetic basis of Jatropha's key attributes such as drought tolerance, the more readily Jatropha improvement will progress."
According to Carlson, Jatropha currently grows best in tropical countries and is already being cultivated as a biofuel on a small scale in India, Southeast Asia and Africa. Breeding a strain that could do well in arid, barren conditions could enable mass cultivation, but large-scale production may still be decades away.
Researchers looked at a little known gene -- JcPIP1 -- because a similar gene in the model plant Arabidopsis is known to play a role in drought response. They also examined JcPIP2, a potential drought response gene in Jatropha identified in 2007 by researchers at Sichuan University. They reported their findings today (July 15) in the Journal of Plant Physiology.
The JcPIP genes code for membrane channels called aquaporins, which are responsible for transporting and balancing water throughout the plant, though exactly how each gene affects aquaporin behavior under environmental stress remains unclear. However, researchers have found that JcPIP1 and JcPIP2 are expressed at different times during a stressful situation, which hints at what roles they play in response and recovery.
By growing unmodified Jatropha samples in conditions simulating high soil salinity and low water availability, the researchers showed that Jatropha was normally more vulnerable and slower to recover from high salinity than from drought conditions.
Using a tobacco mosaic virus to transiently transform Jatropha, the researchers created plants in which JcPIP2 or JcPIP1 was temporarily disabled. They subjected the modified samples to six days of stress and six days of recovery. To gauge the plants' stress responses, they noted physical changes and measured root damage, leaf growth, electrolyte leakage in the leaves, and sap flow and volume.
The researchers found that these stress responses were about the same between the two variants under drought conditions. However, plants with JcPIP1 disabled were slower to recover from salt damage.
Analysis of plant parts during the stress and recovery stages showed that JcPIP2 was mostly active in the early stages of stress while JcPIP1 expression was greater during recovery. The timing indicates that JcPIP1 may be crucial in helping Jatropha recover from damage while JcPIP2 may play a role in prevention.
How the two genes affect other plant functions remains unknown, and how large a part they play in the entire network of drought resistance relies on further study.
"Plants have complex genetic and biochemical pathways for environmental stress resistance, that includes (multiple) genes and pathways," said Carlson. "This inherent redundancy in stress responses ensures survival under varying environmental conditions, and provides many possible approaches to improving resistance."
According to the research team, the next step is to find how the JcPIP genes work at the cellular level, which can provide more detailed profiles of each gene's exact function.
Other researchers on this project include lead investigator Sung Ju Ahn and Ha-Young Jangat, Chonnam National University, Korea; Seong-Wook Yang, associate professor of plant biology and biotechnology, University of Copenhagen; and Yang-Gyu Ku, Wonkwang University, Korea.
The Korea Rural Development Agency, National Research Foundation of Korea and the Korean Ministry of Education, Science and Technology funded this study.
A'ndrea Elyse Messer | EurekAlert!
Ammonium nitrogen input increases the synthesis of anticarcinogenic compounds in broccoli
26.04.2017 | University of the Basque Country
New data unearths pesticide peril in beehives
21.04.2017 | Cornell University
More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.
Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...
Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.
"The structural robustness of thin metal films has significant importance for the reliable operation of smart skin and flexible electronics including...
The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.
Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...
The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...
Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.
Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...
20.04.2017 | Event News
18.04.2017 | Event News
03.04.2017 | Event News
27.04.2017 | Health and Medicine
27.04.2017 | Information Technology
26.04.2017 | Materials Sciences