A team of Agricultural Research Service (ARS) researchers has found a way to speed things up by developing a way to identify genetic markers in the grapevine’s genome that can be linked with specific traits, such as fruit quality, environmental adaptation, and disease and pest resistance.
Computational biologist Doreen Ware, geneticists Edward Buckler and Charles Simon, and research leader Gan-Yuan Zhong have developed a relatively fast and inexpensive way to identify genetic markers not only in grapes, but also in other crops by using modern sequencing approaches. Ware and Buckler work at the ARS Robert W. Holley Center for Agriculture and Health in Ithaca, N.Y.; Simon works at the ARS Plant Genetic Resources Unit at Geneva, N.Y., and Zhong is at the ARS Grape Genetics Research Unit, also at Geneva.
The researchers used the technology to sequence representative portions of the genomes from 10 cultivated grape varieties, six wild varieties and the clone of Pinot Noir originally sequenced by scientists in 2007. They developed filters that allowed them to make corrections for common sequencing errors, and discovered thousands of high-quality single nucleotide polymorphisms, or SNPS, which are genetic markers that can serve as signposts for showing how plants are related to each other.
They then used 9,000 of those SNPs in a custom-designed assay to examine DNA patterns at defined points along each cultivar’s genome. They found the SNPS contained enough data to identify the relationships and geographic origins of the cultivars. The work was published in PLOS One.
Improved technology is expected to make it possible to one day sequence the entire genomes of large numbers of grapes. But in the meantime, the work will help researchers identify portions of the grape genome where they can find genes that confer desirable traits, offering better information for breeders developing new varieties. The technique also should make it easier to identify the origins of other types of plants, characterize relationships in other plant collections, and accelerate genetic mapping efforts in a number of crop species.
ARS is the principal intramural scientific research agency of the U.S. Department of Agriculture. The research supports the USDA priority of promoting international food security.
USDA is an equal opportunity provider, employer and lender. To file a complaint of discrimination, write: USDA, Director, Office of Civil Rights, 1400 Independence Ave., S.W., Washington, D.C. 20250-9410 or call (800) 795-3272 (voice), or (202) 720-6382 (TDD).
Dennis O'Brien | Newswise Science News
Faba fix for corn's nitrogen need
11.04.2018 | American Society of Agronomy
Wheat research discovery yields genetic secrets that could shape future crops
09.04.2018 | John Innes Centre
University of Connecticut researchers have created a biodegradable composite made of silk fibers that can be used to repair broken load-bearing bones without the complications sometimes presented by other materials.
Repairing major load-bearing bones such as those in the leg can be a long and uncomfortable process.
Study published in the journal ACS Applied Materials & Interfaces is the outcome of an international effort that included teams from Dresden and Berlin in Germany, and the US.
Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) together with colleagues from the Helmholtz-Zentrum Berlin (HZB) and the University of Virginia...
Novel highly efficient and brilliant gamma-ray source: Based on model calculations, physicists of the Max PIanck Institute for Nuclear Physics in Heidelberg propose a novel method for an efficient high-brilliance gamma-ray source. A giant collimated gamma-ray pulse is generated from the interaction of a dense ultra-relativistic electron beam with a thin solid conductor. Energetic gamma-rays are copiously produced as the electron beam splits into filaments while propagating across the conductor. The resulting gamma-ray energy and flux enable novel experiments in nuclear and fundamental physics.
The typical wavelength of light interacting with an object of the microcosm scales with the size of this object. For atoms, this ranges from visible light to...
Stable joint cartilage can be produced from adult stem cells originating from bone marrow. This is made possible by inducing specific molecular processes occurring during embryonic cartilage formation, as researchers from the University and University Hospital of Basel report in the scientific journal PNAS.
Certain mesenchymal stem/stromal cells from the bone marrow of adults are considered extremely promising for skeletal tissue regeneration. These adult stem...
In the fight against cancer, scientists are developing new drugs to hit tumor cells at so far unused weak points. Such a “sore spot” is the protein complex...
13.04.2018 | Event News
12.04.2018 | Event News
09.04.2018 | Event News
20.04.2018 | Physics and Astronomy
20.04.2018 | Interdisciplinary Research
20.04.2018 | Physics and Astronomy