A paper published in Science and Technology of Advanced Materials have shown that a synthetic protein called AGMA1 has the potential to promote the adhesion of brain cells in a laboratory setting. It is also cheaper and easier to produce on a large scale. This could help overcome a major challenge in nerve tissue engineering.
Researchers at the University of Milan in Italy have shown that a synthetic protein called AGMA1 has the potential to promote the adhesion of brain cells in a laboratory setting. This could prove helpful in improving cell adhesiveness to biomaterials.
Isolating nerve cells from their original organism and culturing them in the laboratory has long been used as a method to study brain metabolism. It has been challenging, however, to provide brain cell cultures with the necessary “adhesion promoters” that facilitate cell attachment, spreading, growth and morphological development.
Improving cell adhesion to biomaterials is also a major challenge in nerve tissue engineering and is crucial for the development of implanted neural prostheses, such as cochlear implants, and biosensors, such as blood glucose biosensors.
Coating the surfaces of negatively charged cell membranes with positively charged synthetic proteins promotes nerve adhesion and extension in laboratory settings. Most synthetic proteins, however, are toxic to living cells and thus need to be washed off before cell suspensions are spread onto a new plate. They are also unsuitable for applications that are used inside a living organism.
Within the central nervous system, extracellular matrix substances such as collagen and laminin promote the regeneration, differentiation, adhesion and migration of nerve fibers.
A protein sequence found in collagen and laminin has been identified as the minimum sequence that can mediate the adhesion of many cell types, including nerve cells.
AGMA1 is a basic synthetic protein that is biocompatible, water soluble, positively charged, and has a protein sequence similar to that found in collagen and laminin. It is much less toxic to living cells than conventionally used synthetic proteins. AGMA1 is also much easier to prepare on a large scale using relatively low-cost materials. As a result it is much cheaper.
University of Milan scientists tested the potential of AGMA1 to promote the adhesion, proliferation, and differentiation of primary brain cells in the laboratory.
Different primary cell types from rat brain were cultured on AGMA1, and the results compared with those of cells cultured under the same conditions on conventional substrates using other commonly used synthetic proteins. All experimental results showed that the performance of AGMA1 in this respect was comparable to that of conventional substrates.
For further information contact:
Dr Paolo Ferruti
Department of Chemistry
University of Milan
Sci. Technol. Adv. Mater. 15 (2014) 045007
Nagoya physicists resolve long-standing mystery of structure-less transition
21.08.2017 | Nagoya University
Scientists from the MSU studied new liquid-crystalline photochrom
21.08.2017 | Lomonosov Moscow State University
Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.
As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...
Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.
Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...
For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.
While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...
An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.
The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...
A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.
Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...
16.08.2017 | Event News
04.08.2017 | Event News
26.07.2017 | Event News
22.08.2017 | Life Sciences
22.08.2017 | Life Sciences
22.08.2017 | Life Sciences