Genetically engineered fibers of the protein spidroin, which is the construction material for spider webs, has proven to be a perfect substrate for cultivating heart tissue cells, MIPT researchers found. They discuss their findings in an article that has recently come out in the journal PLOS ONE.
The cultivation of organs and tissues from a patient's cells is the bleeding edge of medical research - regenerative methods can solve the problem of transplant rejection. However,it's quite a challenge to find a suitable frame, or substrate, to grow cells on.
These are heart tissue cells grown on a matrix, stained with fluorescent markers.
Credit: © Alexander Teplenin et al. / PLOS ONE
The material should be non-toxic and elastic andshould not be rejected by the body or impede cell growth. A group of researchers led by Professor Konstantin Agladze, who heads the Laboratory of the Biophysics of Excitable Systems at MIPT, works on cardiac tissue engineering. The group has been cultivating fully functional cardiac tissues, able to contract and conduct excitation waves, from cells called cardiomyocytes.
Previously, the group used synthetic polymeric nanofibers but recently decided to assay another material - electrospunfibers of spidroin, the cobweb protein. Cobweb strands are incredibly lightand durable. They're five times stronger than steel, twice more elastic than nylon, and are capable of stretching a third of their length. The structure of spidroin molecules that make up cobweb drag lines is similar to that of the silk protein, fibroin, but is much more durable.
Researchers would normally use artificial spidroin fiber matrices as a substrate to grow implants like bones, tendons and cartilages, as well as dressings. Professor Agladze's team decided to find out whether a spidroin substrate derived from genetically modified yeast cells can serve to grow cardiac cells.
For this purpose, they seeded isolated neonatal rat cardiomyocytes on fiber matrices. During the experiment, the researchers monitored the growth of the cells and tested their contractibility and the ability to conduct electric impulses, which are the main features of normal cardiac tissue.
The monitoring, carried out with the help of a microscope and fluorescent markers, showed that within three to five days a layer of cells formed on the substrate that were able to contract synchronously and conduct electrical impulses just like the tissue of a living heart would.
"We can answer positively all questions we put at the beginning of this research project," Professor Agladze says. "Cardiac tissue cells successfully adhere to the substrate of recombinant spidroin; they grow forming layers and are fully functional, which means they can contract coordinately."
Stanislav Goryachev | EurekAlert!
Hidden talents: Converting heat into electricity with pencil and paper
20.02.2018 | Helmholtz-Zentrum Berlin für Materialien und Energie
Contacting the molecular world through graphene nanoribbons
19.02.2018 | Elhuyar Fundazioa
For the first time, a team of researchers at the Max-Planck Institute (MPI) for Polymer Research in Mainz, Germany, has succeeded in making an integrated circuit (IC) from just a monolayer of a semiconducting polymer via a bottom-up, self-assembly approach.
In the self-assembly process, the semiconducting polymer arranges itself into an ordered monolayer in a transistor. The transistors are binary switches used...
Breakthrough provides a new concept of the design of molecular motors, sensors and electricity generators at nanoscale
Researchers from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague), Institute of Physics of the CAS (IP CAS) and Palacký University...
For photographers and scientists, lenses are lifesavers. They reflect and refract light, making possible the imaging systems that drive discovery through the microscope and preserve history through cameras.
But today's glass-based lenses are bulky and resist miniaturization. Next-generation technologies, such as ultrathin cameras or tiny microscopes, require...
Scientists from the University of Zurich have succeeded for the first time in tracking individual stem cells and their neuronal progeny over months within the intact adult brain. This study sheds light on how new neurons are produced throughout life.
The generation of new nerve cells was once thought to taper off at the end of embryonic development. However, recent research has shown that the adult brain...
Theoretical physicists propose to use negative interference to control heat flow in quantum devices. Study published in Physical Review Letters
Quantum computer parts are sensitive and need to be cooled to very low temperatures. Their tiny size makes them particularly susceptible to a temperature...
15.02.2018 | Event News
13.02.2018 | Event News
12.02.2018 | Event News
20.02.2018 | Life Sciences
20.02.2018 | Life Sciences
20.02.2018 | Physics and Astronomy