Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Bone tissue engineering: Attaching proteins for better regeneration

27.07.2012
Researchers in Japan demonstrate a new protein binding approach for effectively promoting bone regeneration.

Current treatments for bone defects and bone tissue regeneration have significant limitations. Now a new method that immobilises a fusion protein in a hybrid collagen-polymer supportive scaffold shows promise for bone tissue engineering.

Guoping Chen, Yoshihiro Ito and researchers at the Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science, RIKEN, the Immuno-Biological Laboratories Co., Ltd, and the National Institute for Child Health and Development, Tokyo, added a collagen-binding domain from fibronectin to BMP4, a protein that promotes bone regeneration.

The new fusion protein, BMP4-CBD, was used in a scaffold of natural collagen sponge and a strong synthetic polymer, poly(lactic-co-glycolic acid) (PLGA). The scaffold provides space and support for the bone cell growth.

The researchers investigated the BMP4-CBD immobilized on the collagen-PLGA scaffold cultured in vivo for four weeks. They compared the approach with a range of controls including scaffolds with wild-type BMP4 without the collagen-binding domain and scaffolds with just the collagen-binding domain. The expression of specific and non-specific osteogenetic markers used as indicators of bone tissue regeneration was much higher for BMP4-CBD. Using BMP4-CBD also initiated calcification.

“These effects should be attributed to the retention of more effective molecules due to the specific binding of the fusion BMP4 to the collagen,” say the authors. The stimulation effect of the protein promoting the bone regeneration is thus maintained over a longer period. The research may benefit patients suffering from bone defects in the future.

Further information

Publications and Affiliation:

Hongxu Lu1, Naoki Kawazoe1, Takashi Kitajima2, Yuka Myoken3, Masahiro Tomita 3, Akihiro Umezawa4, Guoping Chen1*, Yoshihiro Ito2* Spatial immobilization of bone morphogenetic protein-4 in a collagen-PLGA hybrid scaffold for enhanced osteoinductivity. Biomaterials, 33, 6140–6146, (2012).

1. Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
2. Nano Medical Engineering Laboratory, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako 351-0198, Japan
3. Neosilk Laboratory, Immuno-Biological Laboratories Co., Ltd., 3-13-60 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan
4. National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, Japan

* Corresponding authors


Contact details

International Center for Materials Nanoarchitectonics(WPI-MANA)
1-1 Namiki, Tsukuba-shi Ibaraki, 305-0044 Japan
Email: Guoping Chen, Ph.D Guoping.CHENnims.go.jp
Telephone: +81-29-860-4496

Adarsh Sandhu | Research asia research news
Further information:
http://www.nims.go.jp/mana/

More articles from Materials Sciences:

nachricht One in 5 materials chemistry papers may be wrong, study suggests
15.12.2017 | Georgia Institute of Technology

nachricht Scientists channel graphene to understand filtration and ion transport into cells
11.12.2017 | National Institute of Standards and Technology (NIST)

All articles from Materials Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: First-of-its-kind chemical oscillator offers new level of molecular control

DNA molecules that follow specific instructions could offer more precise molecular control of synthetic chemical systems, a discovery that opens the door for engineers to create molecular machines with new and complex behaviors.

Researchers have created chemical amplifiers and a chemical oscillator using a systematic method that has the potential to embed sophisticated circuit...

Im Focus: Long-lived storage of a photonic qubit for worldwide teleportation

MPQ scientists achieve long storage times for photonic quantum bits which break the lower bound for direct teleportation in a global quantum network.

Concerning the development of quantum memories for the realization of global quantum networks, scientists of the Quantum Dynamics Division led by Professor...

Im Focus: Electromagnetic water cloak eliminates drag and wake

Detailed calculations show water cloaks are feasible with today's technology

Researchers have developed a water cloaking concept based on electromagnetic forces that could eliminate an object's wake, greatly reducing its drag while...

Im Focus: Scientists channel graphene to understand filtration and ion transport into cells

Tiny pores at a cell's entryway act as miniature bouncers, letting in some electrically charged atoms--ions--but blocking others. Operating as exquisitely sensitive filters, these "ion channels" play a critical role in biological functions such as muscle contraction and the firing of brain cells.

To rapidly transport the right ions through the cell membrane, the tiny channels rely on a complex interplay between the ions and surrounding molecules,...

Im Focus: Towards data storage at the single molecule level

The miniaturization of the current technology of storage media is hindered by fundamental limits of quantum mechanics. A new approach consists in using so-called spin-crossover molecules as the smallest possible storage unit. Similar to normal hard drives, these special molecules can save information via their magnetic state. A research team from Kiel University has now managed to successfully place a new class of spin-crossover molecules onto a surface and to improve the molecule’s storage capacity. The storage density of conventional hard drives could therefore theoretically be increased by more than one hundred fold. The study has been published in the scientific journal Nano Letters.

Over the past few years, the building blocks of storage media have gotten ever smaller. But further miniaturization of the current technology is hindered by...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

See, understand and experience the work of the future

11.12.2017 | Event News

Innovative strategies to tackle parasitic worms

08.12.2017 | Event News

AKL’18: The opportunities and challenges of digitalization in the laser industry

07.12.2017 | Event News

 
Latest News

Engineers program tiny robots to move, think like insects

15.12.2017 | Power and Electrical Engineering

One in 5 materials chemistry papers may be wrong, study suggests

15.12.2017 | Materials Sciences

New antbird species discovered in Peru by LSU ornithologists

15.12.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>