Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Bone tissue engineering: Attaching proteins for better regeneration

27.07.2012
Researchers in Japan demonstrate a new protein binding approach for effectively promoting bone regeneration.

Current treatments for bone defects and bone tissue regeneration have significant limitations. Now a new method that immobilises a fusion protein in a hybrid collagen-polymer supportive scaffold shows promise for bone tissue engineering.

Guoping Chen, Yoshihiro Ito and researchers at the Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science, RIKEN, the Immuno-Biological Laboratories Co., Ltd, and the National Institute for Child Health and Development, Tokyo, added a collagen-binding domain from fibronectin to BMP4, a protein that promotes bone regeneration.

The new fusion protein, BMP4-CBD, was used in a scaffold of natural collagen sponge and a strong synthetic polymer, poly(lactic-co-glycolic acid) (PLGA). The scaffold provides space and support for the bone cell growth.

The researchers investigated the BMP4-CBD immobilized on the collagen-PLGA scaffold cultured in vivo for four weeks. They compared the approach with a range of controls including scaffolds with wild-type BMP4 without the collagen-binding domain and scaffolds with just the collagen-binding domain. The expression of specific and non-specific osteogenetic markers used as indicators of bone tissue regeneration was much higher for BMP4-CBD. Using BMP4-CBD also initiated calcification.

“These effects should be attributed to the retention of more effective molecules due to the specific binding of the fusion BMP4 to the collagen,” say the authors. The stimulation effect of the protein promoting the bone regeneration is thus maintained over a longer period. The research may benefit patients suffering from bone defects in the future.

Further information

Publications and Affiliation:

Hongxu Lu1, Naoki Kawazoe1, Takashi Kitajima2, Yuka Myoken3, Masahiro Tomita 3, Akihiro Umezawa4, Guoping Chen1*, Yoshihiro Ito2* Spatial immobilization of bone morphogenetic protein-4 in a collagen-PLGA hybrid scaffold for enhanced osteoinductivity. Biomaterials, 33, 6140–6146, (2012).

1. Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
2. Nano Medical Engineering Laboratory, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako 351-0198, Japan
3. Neosilk Laboratory, Immuno-Biological Laboratories Co., Ltd., 3-13-60 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan
4. National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, Japan

* Corresponding authors


Contact details

International Center for Materials Nanoarchitectonics(WPI-MANA)
1-1 Namiki, Tsukuba-shi Ibaraki, 305-0044 Japan
Email: Guoping Chen, Ph.D Guoping.CHENnims.go.jp
Telephone: +81-29-860-4496

Adarsh Sandhu | Research asia research news
Further information:
http://www.nims.go.jp/mana/

More articles from Materials Sciences:

nachricht Serendipity uncovers borophene's potential
23.02.2017 | Northwestern University

nachricht Switched-on DNA
20.02.2017 | Arizona State University

All articles from Materials Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Breakthrough with a chain of gold atoms

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

Im Focus: DNA repair: a new letter in the cell alphabet

Results reveal how discoveries may be hidden in scientific “blind spots”

Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...

Im Focus: Dresdner scientists print tomorrow’s world

The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.

The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...

Im Focus: Mimicking nature's cellular architectures via 3-D printing

Research offers new level of control over the structure of 3-D printed materials

Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...

Im Focus: Three Magnetic States for Each Hole

Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".

Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Booth and panel discussion – The Lindau Nobel Laureate Meetings at the AAAS 2017 Annual Meeting

13.02.2017 | Event News

Complex Loading versus Hidden Reserves

10.02.2017 | Event News

International Conference on Crystal Growth in Freiburg

09.02.2017 | Event News

 
Latest News

Stingless bees have their nests protected by soldiers

24.02.2017 | Life Sciences

New risk factors for anxiety disorders

24.02.2017 | Life Sciences

MWC 2017: 5G Capital Berlin

24.02.2017 | Trade Fair News

VideoLinks
B2B-VideoLinks
More VideoLinks >>>