Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Bone tissue engineering: Attaching proteins for better regeneration

27.07.2012
Researchers in Japan demonstrate a new protein binding approach for effectively promoting bone regeneration.

Current treatments for bone defects and bone tissue regeneration have significant limitations. Now a new method that immobilises a fusion protein in a hybrid collagen-polymer supportive scaffold shows promise for bone tissue engineering.

Guoping Chen, Yoshihiro Ito and researchers at the Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science, RIKEN, the Immuno-Biological Laboratories Co., Ltd, and the National Institute for Child Health and Development, Tokyo, added a collagen-binding domain from fibronectin to BMP4, a protein that promotes bone regeneration.

The new fusion protein, BMP4-CBD, was used in a scaffold of natural collagen sponge and a strong synthetic polymer, poly(lactic-co-glycolic acid) (PLGA). The scaffold provides space and support for the bone cell growth.

The researchers investigated the BMP4-CBD immobilized on the collagen-PLGA scaffold cultured in vivo for four weeks. They compared the approach with a range of controls including scaffolds with wild-type BMP4 without the collagen-binding domain and scaffolds with just the collagen-binding domain. The expression of specific and non-specific osteogenetic markers used as indicators of bone tissue regeneration was much higher for BMP4-CBD. Using BMP4-CBD also initiated calcification.

“These effects should be attributed to the retention of more effective molecules due to the specific binding of the fusion BMP4 to the collagen,” say the authors. The stimulation effect of the protein promoting the bone regeneration is thus maintained over a longer period. The research may benefit patients suffering from bone defects in the future.

Further information

Publications and Affiliation:

Hongxu Lu1, Naoki Kawazoe1, Takashi Kitajima2, Yuka Myoken3, Masahiro Tomita 3, Akihiro Umezawa4, Guoping Chen1*, Yoshihiro Ito2* Spatial immobilization of bone morphogenetic protein-4 in a collagen-PLGA hybrid scaffold for enhanced osteoinductivity. Biomaterials, 33, 6140–6146, (2012).

1. Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
2. Nano Medical Engineering Laboratory, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako 351-0198, Japan
3. Neosilk Laboratory, Immuno-Biological Laboratories Co., Ltd., 3-13-60 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan
4. National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, Japan

* Corresponding authors


Contact details

International Center for Materials Nanoarchitectonics(WPI-MANA)
1-1 Namiki, Tsukuba-shi Ibaraki, 305-0044 Japan
Email: Guoping Chen, Ph.D Guoping.CHENnims.go.jp
Telephone: +81-29-860-4496

Adarsh Sandhu | Research asia research news
Further information:
http://www.nims.go.jp/mana/

More articles from Materials Sciences:

nachricht Strange but true: Turning a material upside down can sometimes make it softer
20.10.2017 | Universitat Autonoma de Barcelona

nachricht Metallic nanoparticles will help to determine the percentage of volatile compounds
20.10.2017 | Lomonosov Moscow State University

All articles from Materials Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Neutron star merger directly observed for the first time

University of Maryland researchers contribute to historic detection of gravitational waves and light created by event

On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...

Im Focus: Breaking: the first light from two neutron stars merging

Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.

Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....

Im Focus: Smart sensors for efficient processes

Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).

When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...

Im Focus: Cold molecules on collision course

Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.

How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...

Im Focus: Shrinking the proton again!

Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.

It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ASEAN Member States discuss the future role of renewable energy

17.10.2017 | Event News

World Health Summit 2017: International experts set the course for the future of Global Health

10.10.2017 | Event News

Climate Engineering Conference 2017 Opens in Berlin

10.10.2017 | Event News

 
Latest News

Terahertz spectroscopy goes nano

20.10.2017 | Information Technology

Strange but true: Turning a material upside down can sometimes make it softer

20.10.2017 | Materials Sciences

NRL clarifies valley polarization for electronic and optoelectronic technologies

20.10.2017 | Interdisciplinary Research

VideoLinks
B2B-VideoLinks
More VideoLinks >>>