Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Viral 'parasites' may play a key role in the maintenance of cell pluripotency

28.04.2014

In a study published in Nature Genetics, scientists from the RIKEN Center for Life Science Technologies in Japan, in collaboration with the RIKEN Center for Integrative Medical Sciences, the University of Copenhagen and the Joint Genome Institute (Walnut Creek, California) have discovered that "jumping DNA" known as retrotransposons—viral elements incorporated into the human genome—may play a key role in the maintenance of pluripotency, the ability of stem cells to differentiate into many different types of body cells.

This story is part of a fundamental rethinking taking place in genomic science. In 2009, members of the FANTOM Consortium project reported that an important fraction of mammalian transcriptomes—meaning the RNA transcribed from the genome—consists of transcripts derived from retrotransposon elements, vestiges of ancient retroviruses from the same family as HIV that have in the past been considered to only parasite the genome. However, the biological function of these "jumping DNA"–associated RNA transcripts remained unknown.

In the current study on embryonic stem (ES) cells and induced pluripotent stem (iPS) cells using four high-throughput methods including cap analysis gene expression (CAGE), the researchers found that thousands of transcripts in stem cells that have not yet been annotated are transcribed from retrotransposons, presumably to elicit nuclear functions.

These transcripts were found to be expressed in stem cells, but not differentiated cells. Importantly, the work showed that several of these transcripts are involved in the maintenance of pluripotency, since degrading several of them using RNA interference caused iPS cells to lose their pluripotency and differentiate.

... more about:
»HIV »RIKEN »RNA »Science »genes »genomic »mammalian »methods

These transcripts appear to have been recruited, surprisingly both in the human and mouse genome, where they are used to maintain the pluripotency of stem cells. Somehow, organisms including humans appear to have recruited viral elements into their genome in a way that helps to maintain the pluripotency of stem cells that allow them to regenerate. Why this is so remains a mystery for future investigation.

Although the results of the study cannot be put directly into application in regenerative medicine, knowing that retrotransposon elements are essential in the transcriptional control of iPS and ES cells is an essential clue for solving the puzzle of how to create better types of cells in future regenerative medicine studies.

"Our work has just begun to unravel the scale of unexpected functions carried out by retrotransposons and their derived transcripts in stem cell biology. We were extremely surprised to learn from our data that what was once considered genetic 'junk', namely ancient retroviruses that were thought to just parasite the genome, are in reality symbiotic elements that work closely with other genes to maintain iPS and ES cells in their undifferentiated state.

This is quite different from the image given by textbooks that these genomic elements are junk," explains Dr. Piero Carninci, senior investigator of the study.

###

The study was funded by a JSPS (Japan Society for Promotion of Science) grant for Next Generation World-Leading Researchers.

Jens Wilkinson | Eurek Alert!

Further reports about: HIV RIKEN RNA Science genes genomic mammalian methods

More articles from Studies and Analyses:

nachricht Sleeping on Stomach May Increase Risk of Sudden Death in Epilepsy
22.01.2015 | American Academy of Neurology

nachricht Men and Women Process Emotions Differently
21.01.2015 | Universität Basel

All articles from Studies and Analyses >>>

The most recent press releases about innovation >>>

Anzeige

Anzeige

Event News

Better prospects through equal opportunity

26.01.2015 | Event News

2000 Erziehungswissenschaftler an der Uni Kassel erwartet

19.01.2015 | Event News

How are Europe’s landscapes influenced by the changing energy sector?

19.01.2015 | Event News

 
Latest News

How Ionic: Scaffolding Is in Charge of Calcium Carbonate Crystals

27.01.2015 | Materials Sciences

Brain’s On-Off Thirst Switch Identified

27.01.2015 | Life Sciences

Environmental Scientists Find Antibiotics, Bacteria, Resistance Genes in Dust from Feedlots

27.01.2015 | Ecology, The Environment and Conservation

VideoLinks
B2B-VideoLinks
More VideoLinks >>>