The study shows that liquid chromatography-mass spectrometry (LC-MS) can measure variations in histones, which are spool-like proteins that help support and store DNA.
The technology accurately detected differences in the composition of histones in chronic lymphocytic leukemia (CLL) cells compared with their healthy counterparts, immune cells called B lymphocytes.
These variations are a promising molecular biomarker that might improve the diagnosis and gauge response to therapy in CLL patients.
The findings are published online in a recent issue of the journal Proteomics.
"Now that we have validated the technique, we can apply it to study histones as biomarkers that clinicians can use to make decisions about diagnosis and treatment," says principal investigator Michael A. Freitas, a researcher in the Ohio State University Comprehensive Cancer Center's Experimental Therapeutics Program.
Freitas and his colleagues used LC-MS to analyze variations in histones in 40 CLL patients and four healthy patients. It showed significant changes in the relative abundance of histone H2A variants in CLL cells compared with healthy B cells and suggested a correlation between these changes and the severity of CLL.
To verify the accuracy of their measurements, the investigators tested the technology against common sources of error that can arise in clinical testing laboratories. These included analyzing samples prepared in different ways and by different people, and using different instruments. They also re-analyzed samples that were nearly two years old.
"All of these can be huge problems in biomarker analysis," Freitas says. "The danger is that patients can end up being separated into groups based on changes in how the analysis is done rather than on actual changes in the biomarker.
"We still need to be concerned about experimental bias," he says, "but this study demonstrates that we can detect real biological differences in histones in CLL cells, as opposed to methodological differences."
Funding from the Camille and Henry Dreyfus Foundation, the V-Foundation, the National Cancer Institute, the Leukemia and Lymphoma Society and the D. Warren Brown Foundation supported this research.
Darrell E. Ward | EurekAlert!
Obstructing the ‘inner eye’
07.07.2017 | Friedrich-Schiller-Universität Jena
Drone vs. truck deliveries: Which create less carbon pollution?
31.05.2017 | University of Washington
Physicists have developed a new technique that uses electrical voltages to control the electron spin on a chip. The newly-developed method provides protection from spin decay, meaning that the contained information can be maintained and transmitted over comparatively large distances, as has been demonstrated by a team from the University of Basel’s Department of Physics and the Swiss Nanoscience Institute. The results have been published in Physical Review X.
For several years, researchers have been trying to use the spin of an electron to store and transmit information. The spin of each electron is always coupled...
What is the mass of a proton? Scientists from Germany and Japan successfully did an important step towards the most exact knowledge of this fundamental constant. By means of precision measurements on a single proton, they could improve the precision by a factor of three and also correct the existing value.
To determine the mass of a single proton still more accurate – a group of physicists led by Klaus Blaum and Sven Sturm of the Max Planck Institute for Nuclear...
The research team of Prof. Dr. Oliver Einsle at the University of Freiburg's Institute of Biochemistry has long been exploring the functioning of nitrogenase....
A one trillion tonne iceberg - one of the biggest ever recorded -- has calved away from the Larsen C Ice Shelf in Antarctica, after a rift in the ice,...
Physics supports biology: Researchers from PTB have developed a model system to investigate friction phenomena with atomic precision
Friction: what you want from car brakes, otherwise rather a nuisance. In any case, it is useful to know as precisely as possible how friction phenomena arise –...
21.07.2017 | Event News
19.07.2017 | Event News
12.07.2017 | Event News
21.07.2017 | Earth Sciences
21.07.2017 | Power and Electrical Engineering
21.07.2017 | Physics and Astronomy