Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Study reports iron oxide nanoparticles effective for labeling human endothelial cells

21.12.2012
Loading cells too heavily with INOPS can cause cell death

A team of researchers from three medical institutions in Guangzhou, China, have found that iron oxide nanoparticles (INOPS) are a useful contrast agent for in vivo magnetic resonance tracking of transplanted human endothelial cells.

However, the impact of INOPS on the cells varies with a number of factors including the INOPS load. They found that the percentage of iron-labeled cells was significantly lower after 48 hours post-transplantation than at 24 hours post-transplantation. They also found that high INOPS concentration can affect cell activity. High INOPS concentration can induce cell death (apoptosis).

Their study is published in the current issue of Cell Transplantation (21:9), now freely available on-line at http://www.ingentaconnect.com/content/cog/ct/.

"A good MRI contrast agent must possess a number of features," said study co-author Dr. Wen-Li Chen, at South China Normal University's MOE Key Laboratory of Laser Life Science. "Those features are: low toxicity and good stabilization, high sensitivity, good solubility and the ability to remain in the target cell for a long time. In our study, we found that INOPS are sensitive and can perturb the static magnetic field and provide a string change in MR signals."

The researchers found that the INOPS could be taken up by the cell rather than adhering to the exterior of the cell membrane. However, they also found that a proportional relationship might exist between the number of labeled cells and signal intensity.

"For the first time, we identified that autophagy death could take place at high INOPS loading concentrations," said the researchers.

They also discovered that an increased incubation time, from 24 to 48 hours, did not increase cellular uptake of INOPS and that the percentage of labeled cells declined after 24 hours to be significantly lower by 48 hours.

"It is possible that when the intracellular iron becomes saturated, the particles may start to be expelled out of cells," they concluded. "Thus, the determination of optimal loading concentration is an important step in maintaining the quality of cell labeling and cell activity."

Contact: Dr. Wen-Li Chen, MOE Key Laboratory of Laser Life Science, South China Normal University, Guangzhou, China
Tel. +86-20-85224007
Email: chenwl@scnu.ch
Citation: Yang, F-Y.; Yu, M-X.; Zhou, Q.; Chen, W-L.; Gao, P.; Huang, Z. Effects of Iron Oxide Nanoparticle Labeling on Human Endothelial Cells. Cell Transplant. 21(9):1805-1820; 2012.

The Coeditors-in-chief for CELL TRANSPLANTATION are at the Diabetes Research Institute, University of Miami Miller School of Medicine and Center for Neuropsychiatry, China Medical University Hospital, TaiChung, Taiwan. Contact, Camillo Ricordi, MD at ricordi@miami.edu or Shinn-Zong Lin, MD, PhD at shinnzong@yahoo.com.tw or David Eve, PhD at celltransplantation@gmail.com

News release by Florida Science Communications www.sciencescribe.net

Robert Miranda | EurekAlert!
Further information:
http://www.ingentaconnect.com/content/cog/ct/

More articles from Studies and Analyses:

nachricht Rutgers-led innovation could spur faster, cheaper, nano-based manufacturing
14.02.2018 | Rutgers University

nachricht New study from the University of Halle: How climate change alters plant growth
12.01.2018 | Martin-Luther-Universität Halle-Wittenberg

All articles from Studies and Analyses >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Attoseconds break into atomic interior

A newly developed laser technology has enabled physicists in the Laboratory for Attosecond Physics (jointly run by LMU Munich and the Max Planck Institute of Quantum Optics) to generate attosecond bursts of high-energy photons of unprecedented intensity. This has made it possible to observe the interaction of multiple photons in a single such pulse with electrons in the inner orbital shell of an atom.

In order to observe the ultrafast electron motion in the inner shells of atoms with short light pulses, the pulses must not only be ultrashort, but very...

Im Focus: Good vibrations feel the force

A group of researchers led by Andrea Cavalleri at the Max Planck Institute for Structure and Dynamics of Matter (MPSD) in Hamburg has demonstrated a new method enabling precise measurements of the interatomic forces that hold crystalline solids together. The paper Probing the Interatomic Potential of Solids by Strong-Field Nonlinear Phononics, published online in Nature, explains how a terahertz-frequency laser pulse can drive very large deformations of the crystal.

By measuring the highly unusual atomic trajectories under extreme electromagnetic transients, the MPSD group could reconstruct how rigid the atomic bonds are...

Im Focus: Developing reliable quantum computers

International research team makes important step on the path to solving certification problems

Quantum computers may one day solve algorithmic problems which even the biggest supercomputers today can’t manage. But how do you test a quantum computer to...

Im Focus: In best circles: First integrated circuit from self-assembled polymer

For the first time, a team of researchers at the Max-Planck Institute (MPI) for Polymer Research in Mainz, Germany, has succeeded in making an integrated circuit (IC) from just a monolayer of a semiconducting polymer via a bottom-up, self-assembly approach.

In the self-assembly process, the semiconducting polymer arranges itself into an ordered monolayer in a transistor. The transistors are binary switches used...

Im Focus: Demonstration of a single molecule piezoelectric effect

Breakthrough provides a new concept of the design of molecular motors, sensors and electricity generators at nanoscale

Researchers from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague), Institute of Physics of the CAS (IP CAS) and Palacký University...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

2nd International Conference on High Temperature Shape Memory Alloys (HTSMAs)

15.02.2018 | Event News

Aachen DC Grid Summit 2018

13.02.2018 | Event News

How Global Climate Policy Can Learn from the Energy Transition

12.02.2018 | Event News

 
Latest News

Basque researchers turn light upside down

23.02.2018 | Physics and Astronomy

Finnish research group discovers a new immune system regulator

23.02.2018 | Health and Medicine

Attoseconds break into atomic interior

23.02.2018 | Physics and Astronomy

VideoLinks
Science & Research
Overview of more VideoLinks >>>