In a new study, researchers from the Sbarro Health Research Organization Center for Biotechnology Research (SHRO), a cancer, cardiovascular and diabetes research center located in the College of Science and Technology at Temple University in Philadelphia, PA, and at the University of Siena in Siena, Italy examined mechanisms that control Rb2/p130 gene expression in lung fibroblasts and characterize the effects of Rb2/p130 deregulation on the proliferative features of lung cancer cells. Most importantly, their findings reveal why the gene is expressed differently in small and nonsmall lung cancer cells.
The study was funded by SHRO and the Human Health Foundation, a nonprofit biomedical research organization in Terni, Italy. It was published in Molecular Cancer Research.
The new findings disclose the mechanism controlling Rb2/p130 gene expression in lung cells, and that involve two relatively new proteins, CCCTC-binding factor (CTCF) and BORIS (CTCF-paralogue).
"Our research shows that CTCF and BORIS directly regulate Rb2/p130 gene expression in lung cells," says Marcella Macaluso, Ph.D., one of the authors of the study. "We observed that in small lung cancer cells Rb2/p130 exhibits low expression levels, while in non- small lung cancer cells it is overexpressed compared to normal lung cells. However, until now, there were insufficient and conflicting data that did not allow us to precisely link the deregulated expression of Rb2/p130 in lung cancer cells with the genetic mutation of this gene. This study finally disclosed the mechanism and the players controlling Rb2/p130 expression, and these findings have the high potential to provide important information for understanding the proliferative and antiproliferative signals triggered by Rb2/p130."
Also, the research shows that Rb2/p130 is engaged in a complex network of interactions with DNA methyltransferases (DNMTs) and other proteins, including CTCF and BORIS, that are involved in the epigenetic control of chromatin organization and transcription. This complex network of proteins seems to regulate cellular senescence – or aging -- that is a potent anti-cancer mechanism.
"Our studies may provide new insights into the molecular pathways that that are active and correlated to Rb2/p130 expression, new biomarkers for an early diagnosis of lung cancer and/or predictive factors to determine the effect on tumor treatments and insights into the development of therapies based upon clinical modulation of Rb2/p130, CTCF and/or BORIS expression," says Dr. Macaluso.
Future studies are planned to study and decisively dissect the multiple functions of Rb2/p130 in non-small and small cell lung cancer.
Sbarro Health Research Organization Center for Biotechnology Research (www.shro.org) funds the Sbarro Institute for Cancer Research and Molecular Medicine, a leading nonprofit research center for cancer, diabetes, and cardiovascular disease. Based in Philadelphia, Pennsylvania on the campus of Temple University and the University of Siena in Italy, our programs train young scientists from around the globe.
Ilene Raymond Rush | EurekAlert!
Physics of bubbles could explain language patterns
25.07.2017 | University of Portsmouth
Obstructing the ‘inner eye’
07.07.2017 | Friedrich-Schiller-Universität Jena
Spectrally narrow x-ray pulses may be “sharpened” by purely mechanical means. This sounds surprisingly, but a team of theoretical and experimental physicists developed and realized such a method. It is based on fast motions, precisely synchronized with the pulses, of a target interacting with the x-ray light. Thereby, photons are redistributed within the x-ray pulse to the desired spectral region.
A team of theoretical physicists from the MPI for Nuclear Physics (MPIK) in Heidelberg has developed a novel method to intensify the spectrally broad x-ray...
Physicists working with researcher Oriol Romero-Isart devised a new simple scheme to theoretically generate arbitrarily short and focused electromagnetic fields. This new tool could be used for precise sensing and in microscopy.
Microwaves, heat radiation, light and X-radiation are examples for electromagnetic waves. Many applications require to focus the electromagnetic fields to...
Strong light-matter coupling in these semiconducting tubes may hold the key to electrically pumped lasers
Light-matter quasi-particles can be generated electrically in semiconducting carbon nanotubes. Material scientists and physicists from Heidelberg University...
Fraunhofer IPA has developed a proximity sensor made from silicone and carbon nanotubes (CNT) which detects objects and determines their position. The materials and printing process used mean that the sensor is extremely flexible, economical and can be used for large surfaces. Industry and research partners can use and further develop this innovation straight away.
At first glance, the proximity sensor appears to be nothing special: a thin, elastic layer of silicone onto which black square surfaces are printed, but these...
3-D shape acquisition using water displacement as the shape sensor for the reconstruction of complex objects
A global team of computer scientists and engineers have developed an innovative technique that more completely reconstructs challenging 3D objects. An ancient...
26.07.2017 | Event News
21.07.2017 | Event News
19.07.2017 | Event News
28.07.2017 | Health and Medicine
28.07.2017 | Power and Electrical Engineering
28.07.2017 | Life Sciences