Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

SNPs Affect Folate Metabolism in Study of Puerto Rican Adults

11.11.2008
Tufts researchers have linked several single nucleotide polymorphisms (SNPs) in the DNA of Puerto Rican adults to altered concentrations of blood homocysteine and folate and the content of uracil in blood DNA. These measures are indicators of altered folate metabolism, a putative risk factor for many diseases including cancer and neural tube defects.

Researchers at Tufts University have gained further understanding of the genomic basis for altered folate metabolism and the content of uracil in blood DNA.

In a study published in October’s American Journal of Clinical Nutrition, senior author Jimmy Crott, PhD, and colleagues studied nine single nucleotide polymorphisms (SNPs) in five genes involved in folate uptake and retention: folate hydrolase (FOLH1), folate polyglutamate synthase (FPGS), ã-glutamyl hyrdolase (GGH), proton-coupled folate transporter (PCFT), and reduced folate carrier (RFC1) in a cohort of 991 Puerto Rican adults residing in and around Boston. In addition, four SNPs in two genes involved in folate metabolism previously associated with altered blood folate and homocysteine concentrations were studied: methylenetetrahydrofolate reductase (MTHFR) and methionine synthase (MTR).

SNPs are variations in the sequence of nucleotides, or building blocks, that make up genes. Humans possess two copies of every gene. For each SNP, there are three possible genotypes depending on the presence of “normal” and “variant” copies of the gene; two normal copies, one normal and one variant or two variant copies.

Diseases, such as some cancers, have been associated with diminished blood folate concentrations and abnormal folate metabolism. Crott, a scientist in the Vitamins and Carcinogenesis Laboratory at the Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts, and first author Lauren DeVos of Pennsylvania State University found that several SNPs affect folate metabolism, as evidenced by altered concentrations of blood homocysteine, folate, and DNA uracil.

“Perhaps the most intriguing results of this study involve these SNPs that affected the concentration of uracil in DNA. Uracil accumulation has the potential to cause DNA breakage, a mutagenic event that may increase the risk for cancer,” Crott said.

With respect to the MTHFR 677C>T SNP, those with two copies of the variant gene (TT genotype) displayed a 33.8% lower blood DNA uracil content than those with one or less copies of a variant gene (CC and CT genotypes). This observation fits with previous work showing that, under certain conditions, those with the TT genotype have a significantly lower risk for colorectal cancer than CC and CT genotypes. In addition, a significant association was detected between the -124T>G SNP in GGH gene and DNA uracil content. Individuals with one copy of the variant GGH gene (-124TG genotype) had DNA uracil levels 30% higher, while those with two copies of the variant gene (-124GG genotype) had a uracil level 73% higher than wildtypes (-124TT genotype). The authors speculate that this change might feasibly increase these individuals’ risk for cancer.

Differences in blood folate concentrations were also associated with two of the SNPs studied. After correcting for other potentially confounding factors, those with the MTHFR 677TT genotype displayed folate concentrations 4.6% lower than CC genotypes and CT genotypes. Those with at least one copy of the variant T gene of the FOLH1 1561C>T SNP had blood folate concentrations 10.8% higher than those with the CC genotype. “Although the changes we see here are rather modest, low blood folate concentrations are associated with several diseases including colorectal cancer and neural tube defects,” Crott said.

This study was a nested cohort within the ongoing Boston Puerto Rican Health Study (BPRHS). Puerto Rican adults between the ages of 45 and 75 were identified in blocks containing at least 10 Hispanic occupants in the 2000 census, and one per household (randomly selected) interviewed in his or her home. In addition to answering health-related and anthropometric questions, participants filled out a population-validated, food-frequency questionnaire.

“The current study is part of a body of research exploring whether a diet lacking essential nutrients like B vitamins and antioxidants plays a role in health disparities in older Puerto Rican adults,” said co-author Katherine Tucker, a professor at the Friedman School of Nutrition Science and Policy, director of the Dietary Assessment and Epidemiology Research Program at the JM USDA HNRCA, and principal investigator of the BPRHS.

“The BPRHS is building on previous Tufts research which demonstrates that older Puerto Rican adults living in Boston are more likely to develop chronic health conditions such as depression and diabetes compared to older non-Hispanic whites living in the same neighborhoods,” Tucker continued. “We hypothesize these health disparities are triggered by stress associated with poverty, perceived discrimination and poor nutrition among other challenges.”

This work was supported in part by grants from the United States Department of Agriculture, the USDA Agricultural Research Service and the National Institutes of Health.

DeVos L, Chanson A, Liu Z, Ciappio ED, Parnell LD, Mason JB, Tucker KL, Crott JW. American Journal of Clinical Nutrition. 2008 (October); Vol 88, Issue 4. “Associations between single nucleotide polymorphisms in folate uptake and metabolizing genes with blood folate, homocysteine and DNA uracil concentrations.”

About Tufts University School of Nutrition

The Gerald J. and Dorothy R. Friedman School of Nutrition Science and Policy at Tufts University is the only independent school of nutrition in the United States. The school's eight centers, which focus on questions relating to famine, hunger, poverty, and communications, are renowned for the application of scientific research to national and international policy. For two decades, the Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University has studied the relationship between good nutrition and good health in aging populations. Tufts research scientists work with federal agencies to establish the USDA Dietary Guidelines, the Dietary Reference Intakes, and other significant public policies.

Andrea Grossman | Newswise Science News
Further information:
http://www.tufts.edu

More articles from Studies and Analyses:

nachricht The Great Unknown: Risk-Taking Behavior in Adolescents
19.01.2017 | Max-Planck-Institut für Bildungsforschung

nachricht A sudden drop in outdoor temperature increases the risk of respiratory infections
11.01.2017 | University of Gothenburg

All articles from Studies and Analyses >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Traffic jam in empty space

New success for Konstanz physicists in studying the quantum vacuum

An important step towards a completely new experimental access to quantum physics has been made at University of Konstanz. The team of scientists headed by...

Im Focus: How gut bacteria can make us ill

HZI researchers decipher infection mechanisms of Yersinia and immune responses of the host

Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...

Im Focus: Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.

While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...

Im Focus: Studying fundamental particles in materials

Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales

Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...

Im Focus: Designing Architecture with Solar Building Envelopes

Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.

As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Sustainable Water use in Agriculture in Eastern Europe and Central Asia

19.01.2017 | Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

 
Latest News

New Study Will Help Find the Best Locations for Thermal Power Stations in Iceland

19.01.2017 | Earth Sciences

Not of Divided Mind

19.01.2017 | Life Sciences

Molecule flash mob

19.01.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>