Ram Devireddy, assistant professor of mechanical engineering at LSU, recently co-authored an article with Todd Monroe, assistant professor of biological and agricultural engineering, investigating the complex effects of nanoparticles on cell freezing. The report was published in the prestigious journal Nanotechnology.
The results of their study – while not what they expected – could end up impacting cancer treatment. Devireddy and Monroe initiated a study to investigate the effects of gold-based nanoparticles, or microscopic particles equal to one-thousandth the thickness of a single strand of human hair, on cell transport and the response of those cells after being frozen. Their hypothesis: nanoparticles would alleviate the damaging effects generally caused by the freezing process.
“Most cells are like bags of water,” Devireddy said. “Ice crystals have sharp edges that tend to poke the cells and break them up, causing damage. That is why, for example, frozen food, when thawed and cooked, tends to be ‘mushier’ that fresh produce.”
The researchers, along with graduate students Sreedhar Thirumala and Julianne Audiffred, used the nanoparticles to replace dimethylsulfoxide, a commonly used cryoprotective agent.
“Cryoprotective agents, or CPAs, have long been used to alleviate freezing injury and to enhance the number of cells that survive the freezing process,” said Thirumala. The drawback is that CPAs can also cause cell death when used in high concentrations and need to be removed from cells immediately after freezing.
Devireddy and Monroe believed that nanoparticles might act as a benign replacement for CPAs. To test this, they added commercially available gold nanoparticles to cells suspended in a culture medium.
However, contrary to their initial hypothesis, Devireddy and Monroe found that the nanoparticles did not significantly change the freezing response of either HeLa cells, which are derived from a specific cervical cancer cell line, or Jurkat cells, cancer cells commonly used in research due to their abnormally rapid growth rate in lab conditions.
While test results showed that the nanoparticles were not as effective in protecting frozen cells as the more traditional CPAs, there was significant damaging interaction between the nanoparticles and both HeLa and Jurkat cells, suggesting the need for more research.
Potential practical applications for such research includes improved cryosurgical procedures, which are non-invasive procedures used to eradicate cancer tumors inside the body by cooling them to extremely low temperatures.
Both Devireddy and Monroe plan to pursue this project, citing their teamwork as a driving factor in the effectiveness of their research and teaching.
“The benefit of having each other to ‘cross-train’ our students also better prepares them for future careers in bioengineering research,” said Monroe.
Ram Devireddy | EurekAlert!
A sudden drop in outdoor temperature increases the risk of respiratory infections
11.01.2017 | University of Gothenburg
Urbanization to convert 300,000 km2 of prime croplands
27.12.2016 | Mercator Research Institute on Global Commons and Climate Change (MCC) gGmbH
Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.
As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...
At TU Wien, an alternative for resource intensive formwork for the construction of concrete domes was developed. It is now used in a test dome for the Austrian Federal Railways Infrastructure (ÖBB Infrastruktur).
Concrete shells are efficient structures, but not very resource efficient. The formwork for the construction of concrete domes alone requires a high amount of...
Many pathogens use certain sugar compounds from their host to help conceal themselves against the immune system. Scientists at the University of Bonn have now, in cooperation with researchers at the University of York in the United Kingdom, analyzed the dynamics of a bacterial molecule that is involved in this process. They demonstrate that the protein grabs onto the sugar molecule with a Pac Man-like chewing motion and holds it until it can be used. Their results could help design therapeutics that could make the protein poorer at grabbing and holding and hence compromise the pathogen in the host. The study has now been published in “Biophysical Journal”.
The cells of the mouth, nose and intestinal mucosa produce large quantities of a chemical called sialic acid. Many bacteria possess a special transport system...
UMD, NOAA collaboration demonstrates suitability of in-orbit datasets for weather satellite calibration
"Traffic and weather, together on the hour!" blasts your local radio station, while your smartphone knows the weather halfway across the world. A network of...
Fiber-reinforced plastics (FRP) are frequently used in the aeronautic and automobile industry. However, the repair of workpieces made of these composite materials is often less profitable than exchanging the part. In order to increase the lifetime of FRP parts and to make them more eco-efficient, the Laser Zentrum Hannover e.V. (LZH) and the Apodius GmbH want to combine a new measuring device for fiber layer orientation with an innovative laser-based repair process.
Defects in FRP pieces may be production or operation-related. Whether or not repair is cost-effective depends on the geometry of the defective area, the tools...
10.01.2017 | Event News
09.01.2017 | Event News
05.01.2017 | Event News
16.01.2017 | Power and Electrical Engineering
16.01.2017 | Information Technology
16.01.2017 | Power and Electrical Engineering