Tadpoles develop deformed hearts and impaired kidneys and digestive systems when exposed to the widely used herbicide atrazine in their early stages of life, according to research by Tufts University biologists.
The results present a more comprehensive picture of how this common weed killer – once thought to be harmless to animals -- disrupts growth of vital organs in amphibians during multiple growth periods.
In recent years, worldwide amphibian population declines have fueled concerns over the potentially harmful effects of pesticides on "sentinel" organisms. Previous research had revealed negative effects of atrazine on amphibians extremely early and late in development. The Tufts study, published in the February 2008 edition of "Environmental Health Perspectives," examined tadpoles during an often overlooked period of development, organ morphogenesis.
Study Results Broadens Knowledge of Herbicide's Effects During a Vulnerable Stage
Organ morphogenesis is a brief, extremely sensitive phase in the tadpoles' growth cycle when they are starting to develop organs, noted Kelly A. McLaughlin, Associate Professor of Biology and lead researcher in the study. She explained that experiments were designed to broaden the understanding of how chemicals affect biological growth in amphibians over multiple stages of development. A $5,000 Tufts University Faculty Research Marshall Grant helped fund the study.
"Amphibians are very vulnerable to contamination since atrazine is used in the same environs where they live and breed," McLaughlin said.
Atrazine is used to control broadleaf and grassy weeds on golf courses and residential lawns, according to the Federal Environmental Protection Agency. Farmers use it to treat corn and soybeans. Atrazine blocks photosynthesis once it is absorbed by plants. Chronic exposure to the herbicide during metamorphosis altered amphibian gonadal development, according to previous research.
To study the consequences of atrazine exposure during organ morphogenesis, McLaughlin and her colleagues, Professor of Biology J. Michael Reed, doctoral candidate Jenny R. Lenkowski and Lisa Deininger, a Summer Scholars program undergraduate student, collected eggs from adult female frogs and then fertilized them in vitro. Scientists exposed the developing tadpoles to 10, 25 and 35 mg/L of atrazine. The 35 mg/L dosage simulated the average amount of herbicide used when it is applied in the field, said McLaughlin.
Twelve to 24 hours after exposure to atrazine, tadpoles were examined for abnormal heart growth, visceral hemorrhaging, intestinal coiling, edema and apoptosis (normal cell death that is "programmed" by the body).
Compared with control populations, the tadpoles that were exposed to atrazine had a dramatically higher incidence of abnormalities. The degree of deformities generally corresponded to the size of the dose. After 48 hours of exposure, the point at which organ development is disrupted most profoundly, 57 percent of the tadpoles exposed to 35 mg/L of atrazine had hearts that were smaller than normal, compared with 2% to 3% for the two control groups.
Ectopic Cell Death
The Tufts scientists also examined atrazine exposed tadpoles for increased incidence of apoptosis by measuring levels of active caspase-3 in the pronephric kidney and midbrain. Caspase-3 is a protein needed for apoptosis to occur. They conducted measurements after 6, 12, 24 and 48 hours of exposure in tadpoles exposed to 25 and 35 mg/l of atrazine. Researchers observed that the atrazine-exposed tadpoles showed significant increases in caspase-3 levels in the kidney and midbrain at 12 hours and beyond when compared with controls. The findings indicated a high incidence of ectopic, or abnormal, apoptosis.
"The increased levels of apoptosis in the midbrain and pronephric kidney we observe suggest that atrazine may cause tissue malformation by inducing ectopic programmed cell death, either directly or indirectly through a mechanism that has not been identified," wrote the researchers.
McLaughlin and her team hope that their findings will lay a foundation for further research to determine the underlying mechanism by which atrazine exposure can affect so many different organ systems during the same stage of early development.
"Our work here documents that atrazine affects amphibian's early development, so the second question is how is this happening?" she said. "We know it blocks photosynthesis in plants but why does it have such negative impact on amphibians?"
Tufts University, located on three Massachusetts campuses in Boston, Medford/Somerville, and Grafton, and in Talloires, France, is recognized among the premier research universities in the United States. Tufts enjoys a global reputation for academic excellence and for the preparation of students as leaders in a wide range of professions. A growing number of innovative teaching and research initiatives span all Tufts campuses, and collaboration among the faculty and students in the undergraduate, graduate and professional programs across the university's schools is widely encouraged.
Alex Reid | EurekAlert!
Reusable carbon nanotubes could be the water filter of the future, says RIT study
30.03.2017 | Rochester Institute of Technology
Pan-European study on “Smart Engineering”
30.03.2017 | IPH - Institut für Integrierte Produktion Hannover gGmbH
The Institute of Semiconductor Technology and the Institute of Physical and Theoretical Chemistry, both members of the Laboratory for Emerging Nanometrology (LENA), at Technische Universität Braunschweig are partners in a new European research project entitled ChipScope, which aims to develop a completely new and extremely small optical microscope capable of observing the interior of living cells in real time. A consortium of 7 partners from 5 countries will tackle this issue with very ambitious objectives during a four-year research program.
To demonstrate the usefulness of this new scientific tool, at the end of the project the developed chip-sized microscope will be used to observe in real-time...
Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.
The results will be published on March 22 in the journal „Astronomy & Astrophysics“.
Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...
Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.
Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...
In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...
20.03.2017 | Event News
14.03.2017 | Event News
07.03.2017 | Event News
30.03.2017 | Health and Medicine
30.03.2017 | Health and Medicine
30.03.2017 | Medical Engineering