E. coli 0157:H7 was present in tissues of mung bean sprouts and Salmonella in peanut seedlings after the plants' seeds were contaminated with the pathogens prior to planting. Amanda Deering, a postdoctoral researcher in food science, said seeds could be contaminated in such a manner before or after planting through tainted soil or water.
"The pathogens were in every major tissue, including the tissue that transports nutrients in plants," said Deering, whose results were published in separate papers in the Journal of Food Protection and Food Research International.
Deering and Robert Pruitt, a professor of botany and plant pathology, said finding pathogens inside plants has been challenging because tests require slicing off pieces of the plants, which can move the bacteria from the outside to the inside or vice versa. It becomes difficult to know where a pathogen might have been before the plant was cut.
"The results are often imprecise because the methods allow bacteria to move," said Pruitt, a co-author of the findings.
Deering used a fixative to freeze the location of the bacteria in the plant tissues before slicing samples. Antibodies labeled with fluorescent dye were used to detect the pathogens, a process called immunocytochemistry.
"This shows us as close to what was in the plant when it was living as possible," Deering said. "The number of bacteria increased and persisted at a high level for at least 12 days, the length of the studies."
Deering said she was able to count hundreds of bacteria in almost every type of tissue.
Proper sanitization would eliminate Salmonella and E. coli from the surface of foods, but not inner tissues, Deering and Pruitt said. Cooking those foods to temperatures known to kill the pathogens would eliminate them from inner tissues.
Deering and Pruitt will continue to study the pathogens to determine how they survive inside plant tissues and possible ways to eliminate them. The U.S. Department of Agriculture's Agricultural Research Service funded their work.
Abstracts on the research in this release is available at: http://www.purdue.edu/newsroom/research/2011/110815DeeringPathogens.html
Brian Wallheimer | EurekAlert!
WAKE-UP provides new treatment option for stroke patients | International study led by UKE
17.05.2018 | Universitätsklinikum Hamburg-Eppendorf
First form of therapy for childhood dementia CLN2 developed
25.04.2018 | Universitätsklinikum Hamburg-Eppendorf
The more electronics steer, accelerate and brake cars, the more important it is to protect them against cyber-attacks. That is why 15 partners from industry and academia will work together over the next three years on new approaches to IT security in self-driving cars. The joint project goes by the name Security For Connected, Autonomous Cars (SecForCARs) and has funding of €7.2 million from the German Federal Ministry of Education and Research. Infineon is leading the project.
Vehicles already offer diverse communication interfaces and more and more automated functions, such as distance and lane-keeping assist systems. At the same...
A research team led by physicists at the Technical University of Munich (TUM) has developed molecular nanoswitches that can be toggled between two structurally different states using an applied voltage. They can serve as the basis for a pioneering class of devices that could replace silicon-based components with organic molecules.
The development of new electronic technologies drives the incessant reduction of functional component sizes. In the context of an international collaborative...
At the LASYS 2018, from June 5th to 7th, the Laser Zentrum Hannover e.V. (LZH) will be showcasing processes for the laser material processing of tomorrow in hall 4 at stand 4E75. With blown bomb shells the LZH will present first results of a research project on civil security.
At this year's LASYS, the LZH will exhibit light-based processes such as cutting, welding, ablation and structuring as well as additive manufacturing for...
There are videos on the internet that can make one marvel at technology. For example, a smartphone is casually bent around the arm or a thin-film display is rolled in all directions and with almost every diameter. From the user's point of view, this looks fantastic. From a professional point of view, however, the question arises: Is that already possible?
At Display Week 2018, scientists from the Fraunhofer Institute for Applied Polymer Research IAP will be demonstrating today’s technological possibilities and...
So-called quantum many-body scars allow quantum systems to stay out of equilibrium much longer, explaining experiment | Study published in Nature Physics
Recently, researchers from Harvard and MIT succeeded in trapping a record 53 atoms and individually controlling their quantum state, realizing what is called a...
02.05.2018 | Event News
13.04.2018 | Event News
12.04.2018 | Event News
25.05.2018 | Life Sciences
25.05.2018 | Interdisciplinary Research
24.05.2018 | Ecology, The Environment and Conservation