However, these applications require the modification of RNA which otherwise shows poor resistance to cellular nucleases. To this end, typically the ribose 2´-hydroxyl moieties are substituted by O-methyl groups. This step is hampered by the fact that wild-type RNA-Polymerase is inefficient in incorporating modified nucleotides. Although mutant enzymes have been engineered which are able to insert some or even all 2´-O-me-modified nucleotides, none of the enzymes present so far is able to synthesize transcripts of sufficient lengths. By an in-depth analysis of the T7-RNA-Polymerase and protein engineering, the inventors were able to create a variant which is both a generalist polymerase capable of incorporating all 2´-O-me-modified nucleotides as well as synthesizing fully modified RNA of up to approx. 1,000 nucleotides.
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Safety binding system for ski touring and telemarking
20.10.2016 | TechnologieAllianz e.V.
Enantiomerically pure chiral N-acyl-a-aminonitriles - Method for chemical production avoiding the need of toxic reagents
19.10.2016 | TechnologieAllianz e.V.
Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.
"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...
In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.
A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...
By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.
"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...
COMPAMED has become the leading international marketplace for suppliers of medical manufacturing. The trade fair, which takes place every November and is co-located to MEDICA in Dusseldorf, has been steadily growing over the past years and shows that medical technology remains a rapidly growing market.
In 2016, the joint pavilion by the IVAM Microtechnology Network, the Product Market “High-tech for Medical Devices”, will be located in Hall 8a again and will...
'Ferroelectric' materials can switch between different states of electrical polarization in response to an external electric field. This flexibility means they show promise for many applications, for example in electronic devices and computer memory. Current ferroelectric materials are highly valued for their thermal and chemical stability and rapid electro-mechanical responses, but creating a material that is scalable down to the tiny sizes needed for technologies like silicon-based semiconductors (Si-based CMOS) has proven challenging.
Now, Hiroshi Funakubo and co-workers at the Tokyo Institute of Technology, in collaboration with researchers across Japan, have conducted experiments to...
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